B亚型血清学鉴定及基因测序分析  

Serological identification and gene sequencing analysis of B subtype

作　　者：胡思群 朱碎永[1] 施顺秋[1] 钟锦灶 林甲进[1] HU Si-qun;ZHU Sui-yong;SHI Shun-qiu;ZHONG Jin-zao;LIN Jia-jin(The Second Affiliated Hospital&Yuying Childrens Hospital of Wenzhou Medical University,Wenzhou,Zhejiang 325027,China;不详)

机构地区：[1]温州医科大学附属第二医院育英儿童医院,浙江温州325027 [2]平阳县人民医院,浙江平阳325400

出　　处：《中国卫生检验杂志》2019年第23期2837-2839,共3页Chinese Journal of Health Laboratory Technology

基　　金：温州市科技计划项目(Y20170209);贺林院士新医学临床转化工作站科研基金重点项目(18331104);平阳县社会发展科技计划项目(Y2018A35)

摘　　要：目的探讨B亚型血清学鉴定及基因测序分析。方法采用血型血清学方法鉴定ABO血型,采用PCR-SSP、ABO基因分型、基因测序等方法进行分型和序列分析。结果血清学鉴定发现4例B亚型,其中基因型B303/O2 3例、Bw11/02 1例;基因测序发现第3内含子intron3+5 G/A杂合3例、nt695 T/C杂合突变1例。结论采用血型血清学鉴定,结合基因分型技术是正确鉴定ABO亚型的策略。Objective To explore the serological identification and gene sequencing analysis of subtype B. Methods Serological method is performed to identify the ABO blood group, and PCR-SSP, ABO genotyping and gene sequencing are carried out to analyze the genotypes and sequences. Results Four cases of subtype B were found by serological identification, the genotype of which included 3 cases of B303/O2 and 1 case of Bw11/02. 3 cases of intron3+5 G/A heterozygote and 1 case of nt695 T/C heterozygote mutation were revealed by gene sequencing. Conclusion The combination of genotyping and serological identification is the correct strategy for identifying ABO subtypes.

关 键 词：B亚型 血清学 基因序列分析 

分 类 号：R446.ll[医药卫生—诊断学]