羧甲基淀粉钠对红细胞流变特性与氧解离曲线的影响  

Effect of sodium carboxymethyl starch on erythrocyte rheology and oxygen carrying function

作　　者：尤国兴 赵敬湘 王瑛 尹玉静 王权 李鹏龙 戴连宝 施卫群 赵莲 YOU Guo-xing;ZHAO Jing-xiang;WANG Ying;YIN Yu-jing;WANG Quan;LI Peng-long;DAI Lian-bao;SHI Wei-qun;ZHAO Lian(Institute of Health Service and Transfusion Medicine,Academy of Military Medical Sciences,Academy of Military Sciences,Beijing 100850,China;Shanghai Haohui Biotechnology Co.Ltd.,Shanghai 200436,China)

机构地区：[1]军事科学院军事医学研究院卫生勤务与血液研究所,北京100850 [2]上海昊辉生物科技有限公司,上海200436

出　　处：《国际药学研究杂志》2019年第10期755-760,共6页Journal of International Pharmaceutical Research

基　　金：国家重点研发计划资助项目(2018YFC1106503)

摘　　要：目的研究2种相对分子质量的羧甲基淀粉钠(CMS)120、CMS380的理化性质,及其对红细胞流变特性与氧解离曲线的影响。方法根据《中华人民共和国药典》要求,检测CMS120、CMS380、羟乙基淀粉130(HES130)的理化性质,包括pH值、渗透压摩尔浓度和黏度。将生理盐水、CMS130、CMS380和HES130分别与全血7∶3混合(V/V),设为全血组、全血+生理盐水(NSB)组、全血+CMS120(CMS120B)组、全血+CMS380(CMS380B)组和全血+HES130(HES130B)组。检测各组胶体渗透压、血液黏度、红细胞变形与聚集、血氧饱和度50%时氧分压(P50)值。结果不同样品的理化性质检测结果表明,CMS120和CMS380的晶体渗透压摩尔浓度及黏度均显著高于HES130(均为P<0.05)。样品与全血以7∶3(V/V)混合后,CMS380B组和CMS120B组的胶体渗透压较全血组显著提高(均为P<0.05),并显著高于HES130B组(均为P<0.05);在低剪切力(切变率为10/s)下,与全血组比较,NSB组、CMS380B组和CMS120B组全血和血浆黏度显著升高(均为P<0.05),HES130B组全血黏度显著降低(P<0.05);在切变率为10/s、50/s和150/s条件下,CMS120B和CMS380组全血黏度和血浆黏度均显著高于HES130B组(均为P<0.05);在高切变率作用下,CMS380B和HES130B组呈现促进红细胞变形的趋势;CMS120B组和CMS380B组呈促进红细胞聚集趋势,HES130B组显著抑制红细胞聚集幅度(P<0.05);CMS380B组的P50值显著高于HES130B组(P<0.05)。结论CMS120、CMS380能促进红细胞聚集,CMS380使氧释放能力增强。Objective To investigate the physicochemical properties of sodium carboxymethyl starch(CMS) and its effect on erythrocyte rheology and oxygen carrying function.Methods According to the requirements of Chinese Pharmacopoeia,the physicochemical properties were determined for CMS120,CMS380 and hydroxyethyl starch 130(HES130),including pH value,osmotic pressure molar concentration and viscosity.Normal saline,CMS120,CMS380 and HES130 were mixed with whole blood(7∶3,V/V),respectively,and the experimental groups were set as whole blood group,whole blood+normal saline(NSB)group,whole blood+CMS120(CMS120 B)group,whole blood+CMS380(CMS380 B)group,and whole blood+HES130(HES130 B)group.The colloid osmotic pressure,blood viscosity,erythrocyte deformation and aggregation,and the partial pressure of oxygen when blood oxygen saturation was 50%(P50)value were measured for each of these groups.Results The osmotic pressure molar concentration and the viscosity of CMS120 and CMS380 were both significantly higher than those of HES130(all P<0.05)as indicated by the determination of physicochemical properties.Compared with the whole blood group,the colloid osmotic pressure was significantly increased in the CMS380 B and CMS120 B groups(P<0.05),which was also significantly higher than that in the HES130 B group(P<0.05).At low shear rate(10/s),compared with the whole blood group,the whole blood and the plasma viscosities were significantly increased in the NSB,CMS380 B and CMS120 B groups(all P<0.05),but the whole blood viscosity was significantly decreased in the HES130 B group(P<0.05).Under the conditions of shear rate 10/s,50/s and 150/s,compared with the HBS130 B group,the whole blood and the plasma viscosities were both significantly increased in the CMS120 B and CMS380 groups(all P<0.05).At the effect of high shear rate,both the CMS380 and HES130 groups showed a tendency to promote erythrocyte deformation,and the CMS120 B and CMS380 B groups promoted erythrocyte aggregation,while the HES130 B group inhibited erythrocyte aggregati

关 键 词：羧甲基淀粉钠 红细胞聚集 氧解离曲线 血氧饱和度50%时氧分压(P50)值 羟乙基淀粉 

分 类 号：R973.5[医药卫生—药品] R977.8[医药卫生—药学]