木蝴蝶水提物对家兔离体肠平滑肌活动的影响  

Effect of aqueous extract from semen oroxyli on the activity of isolated intestinal smooth muscle of rabbits

作　　者：黄永毅[1] 罗世顺 罗莹[1] 潘勇[3] Huang Yongyi;Luo Shishun;Luo Ying;Pan Yong(Functional Experimental Teaching Center of Youjiang Medical University for Nationalities,Baise 533000,Guangxi,China;Undergraduate of Class 1,Grade 2014,College of Pharmacy,Youjiang Medical University for Nationalities,Baise 533000,Guangxi,China;Teaching and Research Section of Organic Chemistry and Medicinal Chemistry,Youjiang Medical University for Nationalities,Baise 533000,Guangxi,China)

机构地区：[1]右江民族医学院机能实验教学中心,广西百色533000 [2]右江民族医学院药学院2014级本科1班,广西百色533000 [3]右江民族医学院有机化学与药物化学教研室,广西百色533000

出　　处：《右江民族医学院学报》2019年第6期605-609,共5页Journal of Youjiang Medical University for Nationalities

基　　金：广西高校科学技术研究项目(YB2014299)

摘　　要：目的观察木蝴蝶水提物对家兔离体十二指肠平滑肌活动的影响,探讨其作用机制。方法以家兔离体十二指肠平滑肌收缩活动的平均张力和收缩频率为指标,观察木蝴蝶水提物在低、高浓度下对平滑肌的影响;以0.01%乙酰胆碱溶液(先加入)+8 g/L木蝴蝶水提物(后加入);0.01%阿托品溶液(先加入)+8 g/L木蝴蝶水提物(后加入)作用于离体肠平滑肌,并记录平均张力和收缩频率。结果低(4 g/L)、高(8 g/L)浓度的木蝴蝶水提物均能够明显抑制离体小肠平滑肌的收缩活动,平均张力下降(P<0.01),但无浓度依赖性,且对频率无影响;8 g/L木蝴蝶水提物对乙酰胆碱诱导的平滑肌收缩活动有拮抗作用,使平均张力下降(P<0.01);它对阿托品诱导的平滑肌舒张活动有协同作用,进一步促使平均张力下降(P<0.05),对频率均无影响。结论木蝴蝶水提物可减弱家兔离体小肠平滑肌的自发性收缩活动;可明显拮抗乙酰胆碱诱导的平滑肌收缩活动,可协同阿托品诱导的平滑肌舒张活动,对频率无影响。Objective To observe the effect of aqueous extract from semen oroxyli on the activity of isolated duodenal smooth muscle in rabbits,and explore its mechanism.Methods The average tension and contraction frequency of contractile activity of isolated duodenal smooth muscle in rabbits were used as the indexes to observe the effect of low-and high-concentration aqueous extract of semen oroxyli on smooth muscle.The 0.01%acetylcholine solution(added first)+8 g/L aqueous extract of semen oroxyli(added later)and 0.01%atropine solution(added first)+8 g/L aqueous extract of semen oroxyli(added later)were applied on the isolated intestinal smooth muscle,and the average tension and contraction frequency were recorded.Results Both low-concentration(4 g/L)and high-concentration(8 g/L)aqueous extract from semen oroxyli could significantly inhibit the contraction activity of isolated small intestine smooth muscle,and the mean tension decreased(P<0.01),but there was no concentration dependence and no effect on frequency.The 8 g/L aqueous extract of semen oroxyli had an antagonistic effect on acetylcholine-induced smooth muscle contraction activity and decreased the average tension(P<0.01),moreover,it had a synergistic effect on atropin-induced smooth muscle diastolic activity,and could further promote the decrease of average tension(P<0.05),but had no effect on frequency.Conclusion The aqueous extract from semen oroxyli can reduce spontaneous contractile activity of isolated small intestine smooth muscle in rabbits;in addition,it can obviously antagonize the contractile activity of smooth muscle induced by acetylcholine,and cooperate with atropine induced relaxation activity of smooth muscle,but has no effect on the frequency.

关 键 词：木蝴蝶水提物 家兔 离体小肠 平滑肌 乙酰胆碱 阿托品 

分 类 号：R285.5[医药卫生—中药学]