支气管哮喘患儿发作期血清IL-12、IL-6与ET-1、CEC水平的相关性分析  

Study on Correlation of Serum Cytokines IL-12 and IL-6 with ET-1 and CEC Levels in Children with Bronchial Asthma Attack

作　　者：陈璐[1] 陈艳萍[1] 黄建宝[1] 孟燕妮[1] 杨敏[1] 李林瑞[1] 张喜[1] CHEN Lu;CHEN Yan-ping;HUANG Jian-bao(Department of Respiration,Hunan Children's Hospital,Changsha 410007,Hunan)

机构地区：[1]湖南省儿童医院呼吸二科,湖南长沙410007

出　　处：《医学临床研究》2019年第11期2104-2105,2108,共3页Journal of Clinical Research

摘　　要：【目的】探讨支气管哮喘患儿发作期血清白细胞介素-12(IL-12)、IL-6与内皮素-1(ET-1)、循环内皮细胞计数(CEC)水平的相关性。【方法】选取本院诊治的90例支气管哮喘患儿,根据患儿病情程度分为稳定期组和发作期组,每组45例。检测并比较血清IL-12、IL-6、ET-1、CEC水平,并分析发作期患儿血清IL-12、IL-6与ET-1、CEC的相关性。【结果】支气管哮喘患儿发作期血清IL-12水平低于稳定期,血清IL-6及ET-1、CEC水平高于稳定期,其差异均有统计学意义(P<0.05)。L-12、IL-6与ET-1偏相关系数=0.249,P=0.103,提示IL-12、IL-6与ET-1有直线趋势相关性;IL-12、IL-6与CEC偏相关系数=0.340,P=0.024,提示IL-12、IL-6与CEC有直线相关性。【结论】不同分期支气管哮喘患儿血清IL-12、IL-6、ET-1与CEC差异显著,发作期患儿血清IL-12、IL-6与血清ET-1、CEC关系密切,可有效评估患儿病情及指导临床诊治。【Objective】To investigate the correlation of serum cytokines IL-12 and IL-6 with Endothelin-1(ET-1)and Circulating endothelial cells(CEC)in children with bronchial asthma attack.【Methods】A total of 90 children with bronchial asthma were divided into the stable group(45 cases)and the attack group(45 cases)according to asthma attack status.The serum levels of IL-12(the representative cytokine of helper T lymphocyte-1/Th1),IL-6(the representative cytokine of helper T lymphocyte-1/Th2)and ET-1 were measured by ELISA,while the level of CEC was detected by flow cytometry.The levels of Th1/Th2 cytokines(IL-12 and IL-6),ET-1 and CEC were compared between in the two groups.The correlations between serum IL-12 and IL-6 cytokines with levels of ET-1 and CEC were analyzed.【Results】Level of serum IL-12 in asthma attack group was lower than that in the stable group,while serum IL-6,ET-1 and CEC levels in the attack group were higher than those in the stable stage.The differences between the two groups were statistically significant(P<0.05).The partial correlation coefficient of IL-12 and IL-6 with ET-1 was 0.249,P=0.103,suggesting that IL-12 and IL-6 had a linear trend correlation with ET-1.The partial correlation coefficient of IL-12 and IL-6 with CEC was 0.340,P=0.024,suggesting that IL-12 and IL-6 had a linear correlation with CEC.【Conclusion】There are significant differences of serum IL-12,IL-6,ET-1 and blood CEC in children with bronchial asthma at different stages(stable vs attack).Serum cytokines IL-12 and IL-6 are closely related to ET-1 and CEC levels in children with bronchial asthma attack stage,which can effectively evaluate the condition of children and guide clinical diagnosis and treatment.

关 键 词：哮喘持续状态/病理生理学 白细胞介素-6 白细胞介素-12 

分 类 号：R256.12[医药卫生—中医内科学]