甜瓜Dof家族全基因组鉴定与表达分析  

Genome-wide Identification and Expression of Dof Family in Melon(Cucumis melo)

作　　者：张焕欣 李国权[1] 杨惠栋 曹娜 朱方红[1] ZHANG Huanxin;LI Guoquan;YANG Huidong;CAO Na;ZHU Fanghong(Institute of Horticulture,Jiangxi Academy of Agricultural Sciences,Nanchang 330200,China)

机构地区：[1]江西省农业科学院园艺研究所

出　　处：《园艺学报》2019年第11期2176-2187,共12页Acta Horticulturae Sinica

基　　金：国家现代农业产业技术体系建设专项资金项目(CARS-25);公益性行业(农业)科研专项(201503110-05);江西省青年科学基金项目(20192BAB214017);江西省科技支撑计划项目(20141BBF60017);江西省农业科学院创新基金项目(20181CBS002)

摘　　要：基于甜瓜全基因组数据信息,利用生物信息学方法对甜瓜Dof家族基因进行了全面鉴定和系统发育分析,并通过Real-time PCR方法检测了CmDof家族成员对模拟干旱(PEG6000)、盐和低温胁迫的应答。结果表明:在甜瓜中存在34个Dof基因,除1、5和7号染色体外其余染色体均有分布,发生11次染色体片段重复事件和2次串联重复事件,外显子数在1~3之间,其蛋白质等电点在4.95~9.59之间;蛋白序列系统发育分析显示CmDof蛋白可分为9个进化亚群;组织表达模式分析发现该家族基因具有一定的时空表达特异性;多数CmDof基因对PEG6000、盐和低温胁迫有响应,其中CmDof3/CmDof5/CmDof10/CmDof17、CmDof3/CmDof5/CmDof10和CmDof3/CmDof5/CmDof32分别响应PEG6000、盐和低温胁迫。In this study,we systematically investigated the members of Dof family in melon(Cucumis melo L.)based on the whole-genome sequences and elucidated their phylogenetic relationships.Responses to PEG6000,salt and chilling stresses were detected by real-time PCR method.The results showed that a total of 34 CmDof genes were identified in melon.These CmDof genes were distributed on all chromosomes except the 1 st,5 th and 7 th.Two pairs of tandem duplicated genes were identified,and 11 pairs of segmental duplication genes were observed.The number of exons ranged from 1 to 3,and isoelectric points ranged from 4.95 to 9.59.Phylogenetic analysis showed that CmDof proteins were divided into nine phylogenetic subgroups.The expression patterns of CmDof genes in different tissues showed spatiotemporal expression specificity.Additionally,most CmDof genes could respond to PEG6000,salt and chilling stresses.CmDof3/CmDof5/CmDof10/CmDof17,CmDof3/CmDof5/CmDof10 and CmDof3/CmDof5/CmDof32 were responsive to PEG6000,salt and chilling stresses,respectively.

关 键 词：甜瓜 DOF 基因家族 进化分析 表达特性 

分 类 号：S652[农业科学—果树学]