耐药性与敏感性阿萨希毛孢子菌感染小鼠病死率的对比研究  

The comparative analysis of the fatality rate of the mice infected by resistant or senstive T.asahii strains

作　　者：彭卓颖 田艳丽 廖勇 郎德休 敖俊红 杨蓉娅 PENG Zhuo-ying;TIAN Yan-li;LIAO Yong(Institute of Damage and Repair,the Seventh Medical Center of PLA General Hospital,Beijing 100700,China)

机构地区：[1]中国人民解放军总医院第七医学中心全军皮肤损伤修复研究所

出　　处：《实用皮肤病学杂志》2019年第5期263-266,共4页Journal of Practical Dermatology

基　　金：军队“十二五”重点课题项目（BWS11J059）;国家自然科学基金面上项目（81571972）;北京市科技新星项目（Z161100004916139）

摘　　要：目的研究阿萨希毛孢子菌氟康唑体外诱导的耐药株与其亲本菌株的致病性差异。方法通过最低抑菌浓度(MIC)梯度浓度升高的方法诱导出对氟康唑耐药的环境株和临床株,不同菌株感染BALB/c小鼠,观察不同组小鼠病死率的差异。结果成功获得耐药表型稳定的耐药株(氟康唑MIC值>64 μg/ml),通过组织培养和活检组织病理确定建模成功。环境敏感组病死率为(57.50±3.54)%,环境耐药组病死率为(50.00±7.07)%,临床敏感组病死率为(77.50±3.54)%,临床耐药组病死率为(37.50±3.54)%。结论环境敏感株与环境氟康唑诱导的耐药株对小鼠的致病性无明显差异,而临床来源诱导的耐药株宿主体内致病性降低,具体机制需要后续进一步研究。Objective To investigate the pathogenicity differences of parental T.asahii strains CBS8904,CBS2479 and the evolution T.asahii strains which were induced in vitro by fluconazole.Methods Two T.asahii strains,including an environmental isolate CBS8904 and a clinical isolate CBS2479,were subcultured with fluconazole in vitro by the minimum inhibitory concentration(MIC)gradient method,then the different T.asahii strains were used to infect BALB/c mice,and the fatality rate was evaluated.Results Resistant strains 8904R and 2479R were obtained successfully(MIC>64μg/ml).The fatality rates of CBS8904,8904R,CBS2479 and 2479R were(57.50±3.54)%,(50.00±7.07)%,(77.50±3.54)%and(37.50±3.54)%respectively.Conclusion There was no significant difference in pathogenicity between CBS8904 and 8904R,but the fatality rate of the infected mice by 2479R was significantly lower than that by CBS2479.

关 键 词：阿萨希毛孢子菌 氟康唑 体外诱导耐药 病死率 

分 类 号：R379.9[医药卫生—病原生物学]