云南烟草提取物主要生物碱的对比研究  

Comparison of Main Alkaloid from Yunnan Tobacco Extracts

作　　者：张博 宋凌勇 黄世杰 张增辉 郑美玲 朱远洋 ZHANG Bo;SONG Ling-yong;HUANG Shi-jie;ZHANG Zeng-hui;ZHENG Mei-ling;ZHU Yuan-yang(School of Food and Bioengineering,Zhengzhou University of Light Industry,Zhengzhou 450002,China;Technology Center of Guangxi Tobacoo Industry Co.,Ltd.,Nanning 530001,China)

机构地区：[1]郑州轻工业学院食品与生物工程学院,河南郑州450002 [2]广西中烟有限责任公司技术中心,广西南宁530001

出　　处：《化学试剂》2019年第12期1299-1302,共4页Chemical Reagents

摘　　要：为对比分析云南烟草提取物中生物碱含量的差异,建立了气相色谱-质谱联用(GC-MS)方法,并测定4种常见生物碱的含量。结果表明:4种生物碱分离度较高,相关系数≥0. 999 1,相对标准偏差为2. 89%~4. 59%,检出限为0. 29~0. 94μg/m L。在3个加标浓度下,平均回收率为90. 23%~103. 71%。4种生物碱中,重组分含量最高,之后依次是净油、浸膏、轻组分。综合考虑,轻组分效果最佳。To compare the differences in alkaloid of tobacco extracts,tobacco absolute oils,light distilled components of tobacco oil,and heavy distilled components of tobacco oil,gas chromatography-mass spectrometry(GC/MS)method was established and the alkaloids of four common tobacco extracts were determined.The target compounds were well separated,the correlation coefficients were all greater than 0.9991,and the method was reproducible.The relative standard deviation was 2.89%~4.59%.The detection limit of the method was 0.29~0.94μg/mL.The three standard concentrations were high,medium and low.The average recovery rate was 90.23%~103.71%.For nicotine,myosmine,2,3'-bipyridine,cotinine,the highest content of heavy components,followed by the net oil,extract,light components.Comprehensive consideration,the light component works best.

关 键 词：烟草浸膏 烟草净油 轻组分 重组分 气相色谱-质谱联用(GC-MS) 生物碱 

分 类 号：O657.7[理学—分析化学]