抗草甘膦、抗螟虫水稻E1C608的鉴定和重要表型特征分析  

Identification and Analysis of Important Phenotypes of E1C608 with Glyphosate Resistance and Lepidopteran Resistance in Rice

作　　者：曾强 孟秋成[1] 邓力华[1] 李锦江[1] 于江辉[1] 翁绿水[1] 肖国樱[1,3] ZENG Qiang;MENG Qiu-cheng;DENG Li-hua;LI Jin-jiang;YU Jiang-hui;WENG Lu-shui;XIAO Guo-ying(Key Laboratory of Agro-ecological Processes in Subtropical Region,Institute of Subtropical Agriculture,Chinese Academy of Sciences,Changsha 410125,China;University of Chinese Academy of Sciences,Beijing 100049,China;The Innovative Academy of Seed Design,Chinese Academy of Sciences,Beijing 100101,China)

机构地区：[1]中国科学院亚热带农业生态研究所亚热带农业生态过程重点实验室,长沙410125 [2]中国科学院大学,北京100049 [3]中国科学院种子创新研究院,北京100101

出　　处：《中国生物工程杂志》2019年第11期31-38,共8页China Biotechnology

基　　金：国家转基因生物新品种培育科技重大专项(2016ZX08001003-001)资助项目

摘　　要：草害和虫害是造成水稻减产的两个重要因素,培育转基因抗除草剂、抗虫水稻是解决这两个问题的有效途径之一。分子鉴定结果表明,以恢复系R608为受体的遗传转化获得了3个独立转基因事件,其中1个为单拷贝转化体,命名为E1C608-3。用ELISA方法检测外源抗草甘膦和抗螟虫蛋白含量的结果表明,E1C608-3的T2代分蘖期的根、茎、叶中EPSPS蛋白含量为120.16-1223.28μg/g,CRY1C蛋白含量为1.23-8.72μg/g,且两个外源蛋白在不同器官中的表达量均表现为叶>茎>根(P<0.01)。T3代测定结果显示,E1C608-3转化体在秧苗期草甘膦的耐受浓度至少为16g/L,其耐受浓度至少是转化受体R608的16倍。T3代抗虫实验结果显示,E1C608-3对稻纵卷叶螟幼虫平均致死率为95.56%,表现出良好的螟虫抗性。T4代农艺性状测定结果显示,E1C608-3与转化受体存在较大差异,但都处在水稻天然种质资源的变异范围之内。以上结果表明,E1C608-3是一个抗除草剂、抗螟虫的水稻新种质。Weeds and insect pests are two important biotic stresses to result in yield loss in rice,and breeding bioengineered rice with herbicide resistance and insect resistance is one of the effective ways to solve these two problems.And for that,the glyphosate resistance and Lepidopteran resistance as well as molecular and some agronomic characteristics of transgenic line E1C608 in rice were identified.Results of molecular identification showed that three independent transformation events were obtained,in which one single copy insertion event was named as E1C608-3.The transformant E1C608-3 of T2 generation was used to detect exogenous protein contents in root,stem and leaf at tillering stage by ELISA.The protein contents of EPSPS and CRY1C in different organs of E1C608-3 were significantly different both in descending order of leaf>stem>oot(P<0.01),ranged from120.16μg/g to 1223.28μg/g and from 1.23μg/g to 8.72μg/g,respectively.The glyphosate tolerable concentration at seedling stage of E1C608-3 in T3 generation reached at least 16g/L,which was sixteen times higher than that of the transformation receptor R608.The larva mortality of rice leaf roller fed on leaves of E1C608-3 in T3 generation for five days was 95.56%,that showed the E1C608-3 with an excellent resistance to Lepidopteran insects.The data suggested that some agronomic traits were significantly different between E1C608-3 in T4 generation and R608(P<0.01),but all of the variations were within the variation range of natural germplasm resource in rice.Overall,a novel germplasm with glyphosate resistance and Lepidopteran resistance was verified to be developed in rice.

关 键 词：水稻 转基因 抗草甘膦 抗螟虫 农艺性状 

分 类 号：Q819[生物学—生物工程]