不同烧伤程度大鼠血清皮质酮的表达及其意义  

Expression and significance of serum corticosterone in rats with different burn degrees

作　　者：王爽[1] 张静琦[1] 李小兵[1] 樊军[1] 杨雷钧[1] Wang Shuang;Zhang Jingqi;Li Xiaobing;Fan Jun;Yang Leijun(Department of Plastic and Burn Surgery,Tianjin First Center Hospital,Tianjin 300192,China)

机构地区：[1]天津市第一中心医院整形与烧伤外科,300192

出　　处：《中国中西医结合急救杂志》2019年第5期608-610,共3页Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care

基　　金：天津市卫计委科技基金(2015KY11)。

摘　　要：目的观察不同烧伤程度大鼠血清皮质酮及炎症因子的变化.方法选择健康雄性Wistar大鼠150只,按随机数字表法分为正常对照组和中、重度烧伤激素治疗组以及中、重度烧伤生理盐水治疗组,每组30只.麻醉大鼠后复制背部30%Ⅱ、Ⅲ度烫伤模型;正常对照组不进行烫伤、补液和激素治疗.制模后中、重度烧伤激素治疗组腹腔注射氢化可的松200 mg/d;中、重度烧伤生理盐水治疗组腹腔注射生理盐水4 mL·kg^-1·d^-1.于烧伤后1、4、12 h和1、3和7 d检测各组大鼠血清皮质酮、肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)水平.结果中、重度烧伤治疗各组伤后不同时间点血清皮质酮水平均较正常对照组升高,以重度生理盐水治疗组伤后4 h的升高程度较中、重度烧伤激素治疗组和中度烧伤生理盐水治疗组更显著(ng/L:200.45±2.63比110.56±9.23、146.02±3.28、160.02±8.22).除中、重度烧伤激素治疗组伤后1 h外,其余各组不同时间点TNF-α均较正常对照组明显升高(均P<0.05);不同处理方式各组IL-6水平均较正常对照组升高,且以重度烧伤生理盐水治疗组伤后1 d的升高程度较中、重度烧伤激素治疗组和中度烧伤生理盐水治疗组更明显〔TNF-α(ng/L):39.78±4.30比15.99±1.40、20.58±2.10、25.98±1.60,IL-6(ng/L):210.20±6.70比125.45±3.10、149.81±4.20、161.40±3.80,均P<0.05〕. 结论烧伤应激可导致大鼠血清皮质酮水平明显升高,不同程度烧伤后应激剂量氢化可的松对血清皮质酮和炎症因子水平有一定调控作用.Objective To investigate the changes of serum corticosterone and inflammatory factors in rats with different burn degrees.Methods One hundred and fifty male healthy Wistar rats were randomly divided into normal control group,moderate and severe burn hormone treatment groups,and moderate and severe burn physiological saline treatment groups,each group with 30 rats.After anesthesia,the rat models with burnⅡ,Ⅲdegrees of 30%back surface area were replicated;the burn,fluid infusion and steroid therapy were not carried out in the normal control group.After modeling,the moderate and severe burn hormone treatment groups were given intra-abdominal cavity injection of hydrocortisone 200 mg/d;while in the moderate and severe burn normal saline treatment groups,normal saline 4 mL·kg^-1·d^-1 was injected into the abdominal cavity.The dynamic changes of serum corticosterone,tumor necrosis factor-α(TNF-α),and interleukin-6(IL-6)levels were detected at 1,4,12 hours and 1,3 and 7 days after burn injury in each group.Results The levels of serum corticosterone at different time points after moderate and severe burn injury treatment were higher than those in normal control group,the degree of increase at 4 hours after injury in severe burn saline treatment group was more significant than that in moderate and severe burn hormone treatment groups and moderate burn saline treatment group(ng/L:200.45±2.63 vs.110.56±9.23,146.02±3.28,160.02±8.22).Except the level at 1 hour after injury in the moderate and severe burn hormone treatment groups,the TNF-αlevels in all the other groups at different time points were significantly higher than those in the normal control group(all P<0.05);the IL-6 levels in various treatment groups were all higher than the IL-6 level in the normal control group,moreover,the increase degree on 1 day after injury in the severe burn saline treatment group was more obvious than the degrees in the moderate and severe burn hormone treatment groups and the moderate burn saline treatment group[TNF-α(ng/L):3

关 键 词：烧伤 皮质酮 肿瘤坏死因子-Α 白细胞介素-6 

分 类 号：R47[医药卫生—护理学]