肉苁蓉对卵巢早衰大鼠免疫因子和凋亡相关蛋白的影响  

Effect of Cistanche on the expressions of immune factor and apoptosis-related proteins in ovaries of rats with premature ovarian failure

作　　者：刘涛 殷松娜 栾昕[3] LIU Tao;YIN Song-na;LUAN Xin(Teaching and Research Section of Pharmacology,Medical School of Yan'an University,Yan'an 716000,Shannxi Province,China;Department of Physiology,Medical School of Yan'an University,Yan'an 716000,Shannxi Province,China;Department of Anesthesiology,Yan,an People's Hospital,Yan'an 716000,Shannxi Province,China)

机构地区：[1]延安大学医学院药理学教研室,陕西延安716000 [2]延安大学医学院生理学教研室,陕西延安716000 [3]延安市人民医院手术麻醉科,陕西延安716000

出　　处：《中国临床药理学杂志》2019年第23期3084-3087,共4页The Chinese Journal of Clinical Pharmacology

基　　金：陕西省教育厅专项基金资助项目(18JK0869)

摘　　要：目的探讨肉苁蓉对卵巢早衰大鼠免疫因子和凋亡相关蛋白的影响及其作用机制。方法按照体重将SD大鼠随机分为5组:正常组、模型组和低、中、高3个剂量实验组,每组10只。用雷公藤多苷50μg·g^-1给予大鼠制备卵巢早衰大鼠模型。低、中、高3个剂量实验组分别给予5,10,20 g·kg^-1肉苁蓉。用酶联免疫吸附法检测大鼠促卵泡激素(FSH)、雌激素和抗苗勒管激素(AMH)水平;以实时定量聚合酶链反应检测肿瘤坏死因子-α(TNF-α)和干扰素-γ(IFN-γ)的基因相对表达水平;以免疫印迹法检测B淋巴细胞瘤-2(Bcl-2)和B淋巴细胞瘤-2相关X蛋白(Bax)蛋白相对表达水平。结果正常组、模型组和低、中、高3个剂量实验组的FSH水平分别为(16.42±1.76),(31.83±2.67),(25.89±2.48),(23.73±2.05)和(21.55±2.94)U·L^-1;上述这5组的雌激素水平分别为(97.64±7.85),(53.47±6.98),(89.56±7.28),(93.35±6.29)和(92.45±5.60)ng·L^-1;上述这5组的AMH水平分别为(280.84±22.42),(149.35±14.57),(231.71±27.38),(253.33±27.36)和(265.64±25.34)pg·mL^-1;上述这5组的TNF-α基因的相对表达水平分别为1.00±0.00,3.54±0.35,2.41±0.27,2.32±0.25和2.13±0.21;上述这5组的IFN-γ基因的相对表达水平分别为1.00±0.00,4.45±0.98,2.93±0.31,2.85±0.29和2.67±0.26;上述这5组的Bcl-2蛋白的相对表达水平分别为0.72±0.05,0.28±0.04,0.42±0.04,0.51±0.06和0.64±0.07;上述这5组的Bax蛋白的相对表达水平分别为0.48±0.04,1.05±0.07,0.86±0.08,0.69±0.07和0.53±0.06。模型组与正常组比较,或者低、中、高3个剂量实验组与模型组比较,差异均有统计学意义(均P<0.05)。结论肉苁蓉能够调控FSH、雌激素和AMH的水平,抑制TNF-α和IFN-γ基因的表达,其可能通过调控性激素因子及Bcl-2/Bax蛋白的平衡从而影响卵巢早衰。Objective To investigate the effect of Cistanche deserticola on the expression of immune factor and apoptosis-related proteins in rats with premature ovarian failure and its mechanism.Methods SD rats were divided into 5 groups:normal group,model group,and low,medium,high doses experimental groups,10 rats in each group.The premature ovarian failure rats model were prepared by using 50μg·g^-1 tripterygium.The low,medium,high doses experimental groups were treated with 5,10,20 g·kg^-1 Cistanche respectively in model rats.Thelevels of follicle stimulating hormone(FSH),estrogen and anti-mullerian hormone(AMH)were detected by enzyme-linked immunosorbent assay.The relative expressions of tumor necrosis factor-α(TNF-α)and interferon-γ(IFN-γ)mRNA were detected by real-time quantitative polymerase chain reaction.The relative expressions of B lymphocyte-2(Bcl-2)and B lymphoma-2 related X(Bax)proteins were detected by Western blotting.Results The levels of FSH in normal group,model group and low,medium,high doses experimental groups were(16.42±1.76),(31.83±2.67),(25.89±2.48),(23.73±2.05)and(21.55±2.94)U·L^-1,respectively;the levels of estrogen in the above five groups were(97.64±7.85),(53.47±6.98),(89.56±7.28),(93.35±6.29)and(92.45±5.60)ng·L^-1,respectively;the levels of AMH in the above five groups were(280.84±22.42),(149.35±14.57),(231.71±27.38),(253.33±27.36)and(265.64±25.34)pg·mL^-1,respectively;the relative expression levels of TNF-αmRNA in the above five groups were 1.00±0.00,3.54±0.35,2.41±0.27,2.32±0.25,2.13±0.21,respectively;the relative expression levels of IFN-γmRNA in the above five groups were 1.00±0.00,4.45±0.98,2.93±0.31,2.85±0.29,2.67±0.26,respectively;the relative expression levels of Bcl-2 protein in the above five groups were 0.72±0.05,0.28±0.04,0.42±0.04,0.51±0.06,0.64±0.07,respectively;the relative expression levels of Bax protein in the above five groups were 0.48±0.04,1.05±0.07,0.86±0.08,0.69±0.07,0.53±0.06,respectively.Comparing between model group and no

关 键 词：肉苁蓉 卵巢早衰 免疫因子 B淋巴细胞瘤-2 B淋巴细胞瘤-2相关X蛋白 

分 类 号：R28[医药卫生—中药学]