乙型肝炎病毒体外感染和复制的细胞模型  

Cell models for studying HBV infection and replication in vitro

作　　者：王明凤 曹佳莉 袁权[2] 夏宁邵[2] Mingfeng Wang;Jiali Cao;Quan Yuan;Ningshao Xia(College of Life Sciences,Xiamen University,Xiamen 361102,Fujian Province,China;National Institute of Diagnostics and Vaccine Development in Infectious Diseases,Xiamen University,Xiamen 361102,Fujian Province,China)

机构地区：[1]厦门大学生命科学学院,福建厦门361102 [2]厦门大学国家传染病诊断试剂与疫苗工程技术中心,福建厦门361102

出　　处：《微生物学报》2019年第12期2263-2275,共13页Acta Microbiologica Sinica

基　　金：国家自然科学基金(81672023)~~

摘　　要：慢性乙型肝炎病毒(Hepatitis B virus,HBV)感染是严重威胁人类生命健康的世界性公共卫生问题。基于现有抗HBV药物的治疗策略,仅能在极少部分患者中实现慢性乙肝的功能性治愈。发展更为有效的抗HBV药物,需要更加透彻全面地认识各个病毒组分和关键宿主因子在HBV感染和复制生命周期中发挥的功能和机制,并在此基础上发现鉴定新的治疗靶点。支持HBV体外感染和复制的细胞模型,是研究HBV生活史的重要工具,并在治疗新靶点的发现和候选药物功效评估等研究工作中发挥关键作用。本文对支持HBV感染和复制细胞模型的新近研究进展进行梳理分析,并对这些模型的应用特点和局限性、新近研究进展和未来发展方向进行系统阐述和讨论。Chronic hepatitis B virus(HBV)infection is a worldwide public health problem that poses a serious threat to human health.Currently,only a very small fraction of patients can achieve functional cure based on the existing treatment strategies of anti-HBV drugs.The development of more effective drugs against HBV certainly requires a more comprehensive understanding on the roles and mechanisms of each viral component and its related host factors in viral life cycle,and therefore providing scientific clues for further identification of novel therapeutic targets.In vitro cell models supporting HBV replication and infection are important tools for basic researches of HBV life cycle,and play essential roles in the identification of novel anti-HBV targets and efficacy evaluation of drug candidate.In this review,we summarize the recent research advances on the cell culture models supporting HBV infection and replication,and systematically illustrate and discuss their application characteristics and limitations and highlight perspectives for further developments.

关 键 词：乙型肝炎病毒 复制 感染 细胞模型 

分 类 号：R51[医药卫生—内科学]