中华眼镜蛇血清对自身蛇毒中主要酶类活性的抑制作用  

Inhibitory Effect of Naja atra Serum on the Activities of Major Enzymes from Its Venom

作　　者：王萍 李恒[2] 和七一[1] 胡先成[1] 熊艳 彭建军[1] 余晓东[1] WANG Ping;LI Heng;HE Qiyi;HU Xiancheng;XIONG Yan;PENG Jianjun;YU Xiaodong(Chongqing Key Laboratory of Animal Biology,Chongqing Engineering Research Center of Bioactive Substance,Ministry of Education Engineering Research Center of Active Substance and Biotechnology,College of Life Sciences,Chongqing Normal University,Chongqing 401331;Institute of Forensic Science,Criminal Police Crops of Chongqing Public Security Bureau,Chongqing 400021,China)

机构地区：[1]重庆师范大学生命科学学院教育部活性物质生物技术工程研究中心重庆市生物活性物质工程研究中心,动物生物学重庆市市级重点实验室,重庆401331 [2]重庆市公安局物证鉴定中心,重庆400021

出　　处：《重庆师范大学学报（自然科学版）》2019年第6期132-138,F0003,共8页Journal of Chongqing Normal University:Natural Science

基　　金：国家自然科学基金（No.31470570）;重庆市科技局社会事业与民生保障科技创新专项（No.cstc2017shms-xdny80077）

摘　　要：【目的】探究中华眼镜蛇(Naja atra)血清对自身蛇毒中主要酶类活性的抑制作用。【方法】不同稀释倍数的中华眼镜蛇血清与蛇毒于37℃孵育30min后,检测蛇毒中磷脂酶A2、透明质酸酶、乙酰胆碱酯酶、L-氨基酸氧化酶和5′-核苷酸酶活性的变化情况。【结果】中华眼镜蛇血清对自身蛇毒中磷脂酶A2和5′-核苷酸酶具有明显的抑制作用:当血清稀释2倍时,可以使蛇毒磷脂酶A2活性降低28.44%(p<0.001);当血清稀释8倍时,可以使蛇毒中5′-核苷酸酶活性活性降低23.44%(p<0.001)。中华眼镜蛇血清对自身蛇毒中的透明质酸酶、乙酰胆碱酯酶和L-氨基酸氧化酶均无抑制作用。【结论】中华眼镜蛇血清中存在蛇毒磷脂酶A2和5′-核苷酸酶的抑制剂,该结果为后期蛇毒抑制剂的筛选提供了理论依据。[Purposes]To investigate the inhibitory effect of Naja atra serum on the activities of major enzymes from its venom.[Methods]The activities of phospholipase A2,hyaluronidase,acetylcholinesterase,L-amino acid oxidase and 5′-nucleotidase from N.atravenom were detected after preincubation the venom with a series of diluted serum samples at 37℃for 30 min.[Findings]The N.atra serum significantly inhibited the activities of phospholipase A2 and 5′-nucleotidase from its venom.The activity of phospholipase A2 was decreased by 28.44%(p<0.001)when serum was diluted by 2 times.The 5′-nucleotidase activity was decreased by 23.44%(p<0.001)when the serum was diluted by 8 times.However,the serum did not inhibit the activities of hyaluronidase,acetylcholinesterase and L-amino acid oxidase from N.atravenom.[Conclusions]There are inhibitors of phospholipase A2 and 5′-nucleotidase of N.atravenom in its serum,which provides a theoretical basis for the screening of venom inhibitors.

关 键 词：中华眼镜蛇 血清 蛇毒 抑制作用 

分 类 号：Q599[生物学—生物化学]