金钗石斛破壁粉对裸鼠人肝癌细胞HepG2移植瘤生长的抑制作用  

Inhibitory Effect of Dendrobium nobile Lindl Wall-broken Powder on Tumor Growth of Transplanted Human Liver Cancer Cell Line HepG2 in Nude Mice

作　　者：贺凯 张丹 李小芳 刘睿涵 黄健麟 刘明华 HE Kai;ZHANG Dan;LI Xiao-fang;LIU Rui-han;HUANG Jian-lin;LIU Ming-hua(Department of Hepatobiliary Surgery,Affiliated Hospital of Southwest Medical University,Luzhou 646000,China;Department of Pharmacology,School of Pharmacy,Southwest Medical University,Luzhou 646000,China;Sichuan College of Traditional Chinese Medicine,Mianyang 621000,China)

机构地区：[1]西南医科大学第一附属医院肝胆外科,四川泸州646000 [2]西南医科大学药学院药理教研室,四川泸州646000 [3]四川中医药高等专科学校,四川绵阳621000

出　　处：《中国药学杂志》2019年第22期1865-1870,共6页Chinese Pharmaceutical Journal

基　　金：四川省教育厅重点项目资助(17ZA0429);泸州市-沪医联合资助项目(2013LZLY-J13)

摘　　要：目的研究金钗石斛破壁粉对人肝癌细胞HepG2裸鼠移植瘤生长及肿瘤血管生成的抑制作用。方法复制裸鼠人肝癌细胞HepG2移植瘤模型,随机分为阴性对照组(生理盐水)、阳性对照组(贝伐单抗注射液,5 mg·kg^-1,腹腔注射给药)和金钗石斛破壁粉低、中、高3个剂量组(50,100和200 mg·kg^-1,灌胃给药),给药结束后剥取瘤体并称重,常规石蜡切片,采用免疫组化法分析瘤体内微血管密度(microvessel density,MVD)和血管内皮生长因子(vascular endothelial growth factor,VEGF)表达的影响。结果金钗石斛破壁粉治疗组给药后肿瘤体积(TV)、相对肿瘤体积(RTV)和相对肿瘤增殖率[T/C(%)]均明显下降,其中[T/C(%)]分别为60.56%、48.59%和47.15%;金钗石斛破壁粉治疗组微血管分布较稀疏,MVD值由对照组的(65.67±12.05)分别减少至(51.53±10.75)、(45.89±9.24)和(40.76±8.93)。金钗石斛破壁粉治疗组阳性表达率由对照组的(82.25±15.59)%下降至(65.27±13.88)%、(48.02±12.14)%和(44.07±10.34)%。结论金钗石斛破壁粉能明显抑制裸鼠HepG2移植瘤的生长,可能与其下调瘤组织VEGF的表达和降低移植瘤微血管密度MVD有关。OBJECTIVE To study the inhibitory effect of Dendrobium nobile Lindl wall-broken powder on tumor growth and angiogenesis of transplanted human liver cancer cell line HepG2 in nude mice.METHODS The transplant tumor model in nude mice for HepG2 was established.All tumor-bearing nude mice were randomly divided into control group(normal saline),positive group(bevacizumab,5 mg·kg^-1,intraperitioneally),and low-,medium-,high-dose Dendrobium nobile Lindl wall-broken powder-treated groups(50,100 and 200 mg·kg^-1,intragastrically).The tumor xenografts were harvested and measured for their weights.Immunohistochemistry was used to detected microvessel density(microvessel density,MVD)and vascular endothelial growth factor(vascular endothelial growth factor,VEGF)expression.RESULTS After the administration,TV(tumor volume),RTV(relative tumor volume),and T/C(%)in Dendrobium nobile Lindl wall-broken powder treatment group decreased significantly,T/C(%)was 60.56%,48.59%,and 47.15%,respectively.SABC assay showed that MVD in Dendrobium nobile Lindl wall-broken powder treatment group was sparse,MVD value decreased from(65.67±12.05)to(51.53±10.75),(45.89±9.24)and(40.76±8.93),Dendrobium nobile Lindl wall-broken powder can significantly reduce the positive expression of VEGF.The positive expression rate reduced from(82.25±15.59)% to(65.27±13.88)%,(48.02±12.14)% and(44.07±10.34)%.CONCLUSION Dendrobium nobile Lindl wall-broken powder can inhibit tumor growth of transplanted liver cancer cell line HepG2 in nude mice in varying degrees,which may be related to reducing MVD value and the expression of VEGF in tumor tissue.

关 键 词：金钗石斛 破壁粉 HEPG2 微血管密度 血管生成 

分 类 号：R285[医药卫生—中药学] R979.1[医药卫生—中医学]