龙岩市鼠伤寒沙门菌分子分型、毒力基因及耐药性测定  

Molecular typing,virulence genes and drug resistance of Salmonella typhimurium in Longyan city

作　　者：陈前进[1,2] 李美华[1] 曹春远[1] 廖琳虹[1] 陈海滨[1] 陈小东 Chen Qianjin;Li Meihua;Cao Chunyuan;Liao Linhong;Chen Haibin;Chen Xiaodong(Department of Clinical Laboratory in Center for Disease Control and Prevention of Longyan City,Longyan 364000,China;Department of Clinical Laboratory,Teaching Base of Fujian Medical University in Center for Disease Control and Prevention of Longyan City,Longyan 364000,China)

机构地区：[1]龙岩市疾病预防控制中心检验科,364000 [2]福建医科大学龙岩市疾病预防控制中心教学点检验科,364000

出　　处：《中华微生物学和免疫学杂志》2019年第11期805-811,共7页Chinese Journal of Microbiology and Immunology

基　　金：福建省卫生厅青年科研课题项目(2014-1-96);2014年龙岩市第二批科技项目(2014LY29);2015—2016年福建卫生计生系统有突出贡献中青年专家工作经费资助(闽财社指[2017]52号)。

摘　　要：目的分析龙岩市鼠伤寒沙门菌脉冲场凝胶电泳(PFGE)分子分型特点、耐药特征和毒力基因的携带情况,为制定防治措施提供参考。方法收集自2010—2017年散发腹泻病例、食物中毒和生禽肉中分离的鼠伤寒沙门菌79株。进行PFGE分析并测定对15种常用药物的最小抑菌浓度(minimal inhibitory concentration,MIC),同时对其中55株用PCR方法检测沙门菌9个毒力基因(sopB、invA、sifA、sscA、sseE、spvB、pefA、spvR、spvC)片段。结果79株鼠伤寒沙门菌分为61个PFGE型别,其中P1、P3和P21型分别包含10、3和4株。P1带型中的7株来源于1起食物中毒事件。按照85%的分类标准,79株鼠伤寒沙门菌可分为5个优势基因簇(G1~G5)。79株鼠伤寒沙门菌药敏结果以氨苄西林耐药率最高(88.61%),其次是四环素(87.34%)和链霉素(73.41%)。耐3种或3种以上抗菌药物的多重耐药菌占84.81%(67/79)。进行毒力基因检测的全部55株菌均检出invA、sopB、sseE、sscA,其中54株检出sifA,31株(56.4%)检出spvC,分别有10、11、12株检出spvB、spvR、pefA(18%~22%)。检出5种或6种毒力基因的菌株占76.4%(42/55),均为invA、sopB、sseE、sscA、sifA阳性,spvB、spvR、pefA阴性,检出全部9种毒力基因的菌株占18.2%(10/55)。结论龙岩市鼠伤寒沙门菌PFGE型别呈现多态性,存在3个优势PFGE型别簇。在食物中毒事件中,PFGE分子分型检测能快速预警鼠伤寒沙门菌散在暴发和溯源。鼠伤寒沙门菌多重耐药现象应引起重视,需加强对多重耐药株的监控及抗菌药物使用的监管。鼠伤寒沙门菌携带毒力基因数量较多,毒力较强。Objective To analyze the molecular typing characteristics by pulsed field gel electrophoresis(PFGE),drug resistance and virulence genes of Salmonella typhimurium in Longyan city in order to provide reference for the prevention and control.Methods A total of 79 Salmonella typhimurium strains were isolated from sporadic cases of diarrhea and food poisoning and raw poultry meat samples during 2010 to 2017.PFGE was performed to measure the minimum inhibitory concentrations(MIC)of 15 commonly used drugs against them.PCR was used to detect nine virulence genes(sopB,invA,sifA,sscA,sseE,spvB,pefA,spvR,spvC)in 55 strains.Results The 79 Salmonella typhimurium strains belonged to 61 PFGE types.There were 10,three and four strains of P1,P3 and P21 types,respectively.Seven P1 type strains were isolate from one food poisoning event.According to the 85%classification standard,79 Salmonella typhimurium strains could be divided into five predominant gene clusters(G1-G5).Drug susceptibility test showed that the 79 strains had the highest resistance rate to ampicillin(88.61%),followed by that to tetracycline(87.34%)and streptomycin(73.41%).Multidrug resistant bacteria resistant to three or more antibacterial drugs accounted for 84.81%(67/79).All of the 55 strains carried invA,sopB,sseE and sscA genes.The other five genes,sifA,spvC,spvB,spvR and pefA,were detected in 54,31.10,11 and 12 strains,respectively.There were 76.4%(42/55)of the strains carrying five or six virulence genes and all were positive for invA,sopB,sseE,sscA and sifA,and negative for spvB,spvR and pefA.The strains carrying all of the nine virulence genes accounted for 18.2%(10/55).Conclusions Salmonella typhimurium strains isolated in Longyan city had a diverse PFGE type.P1,P3 and P21 types were the three predominant PFGE types.In the food poisoning event,PFGE molecular typing could quickly alert the outbreak and traceability of Salmonella typhimurium.Attention should be paid to the multidrug resistance in Salmonella typhimurium.Monitoring of multidrug-resistant str

关 键 词：鼠伤寒沙门菌 PFGE分子分型 聚类分析 最小抑菌浓度 多重耐药 毒力基因 沙门菌毒力岛 沙门菌质粒毒力基因 

分 类 号：R51[医药卫生—内科学]