纳米乳剂作为疫苗佐剂可增强老龄鼠和年轻鼠的抗流感体液免疫  

Nano-emulsion as a vaccine adjuvant can enhance the humoral immunity against influenza in aged and young mice

作　　者：单璞 王志标 魏多前 郝少杰 陈德祥 徐静 Shan Pu;Wang Zhibiao;Wei Duoqian;Hao Shaojie;Chen Dexiang;Xu Jing(The Third Laboratory,National Vaccine&Serum Institute,Beijing 101111,China;Chengdu Maikekang BioTechnology Co.,Ltd.,Chengdu 610041,China)

机构地区：[1]国药中生生物技术研究院有限公司第三研究室,北京101111 [2]成都迈科康生物科技有限公司,610041

出　　处：《中华微生物学和免疫学杂志》2019年第11期869-874,共6页Chinese Journal of Microbiology and Immunology

摘　　要：目的评价纳米乳剂(nano-emulsion adjuvant,NE)作为疫苗佐剂联合H1N1流感病毒裂解疫苗对老龄鼠与年轻鼠体液免疫的增强作用。方法以纳米乳剂配伍流感裂解H1N1疫苗对老龄鼠与年轻鼠肌肉注射免疫,免疫剂量血凝素(hemagglutinin,HA)0.012μg/只,两针免疫间隔28 d。初免后27 d检测血清中血凝抑制(hemagglutination inhibition,HI)效价,末次免疫后14 d检测血清中HI效价、IgG1和IgG2a抗体水平,研究其对老龄鼠和年轻鼠的佐剂增强作用,同时设无佐剂及生理盐水(NS)对照组。纳米乳剂配伍流感裂解H1N1疫苗免疫小鼠,末次免疫后14 d以10倍病毒半数致死量(median lethal dose,LD50)A/Puerto Rico/8/34(H1N1)流感毒株进行攻毒试验,连续14 d观察小鼠体重和生存率的变化。结果纳米乳剂联合HA可诱导较单独HA肌注更强的血清HI、IgG1抗体水平和HI抗体阳转率,在老龄鼠中的免疫增强作用更为明显。攻毒保护试验结果显示,纳米乳剂能够增强HA免疫原性以及小鼠对鼠肺适应株A/Puerto Rico/8/34(H1N1)攻击的抵抗力。结论纳米乳剂可增强流感抗原的免疫原性,且对老龄鼠的增强作用更为明显。Objective To evaluate the immunogenicity of split influenza H1N1 vaccine formulated with an oil-in-water nano-emulsion adjuvant in aged mice and young mice.Methods A nano-emulsion adjuvant formulated split influenza H1N1 vaccine was used to immunize aged and young mice through intramuscular injection.Each mouse was immunized with 0.012μg of hemagglutinin(HA)twice with an interval of 28 d.Hemagglutination inhibition(HI)titers in serum were measured 27 d after first immunization.Serum HI,IgG1 and IgG2a titers were detected 14 d after the last immunization.No adjuvant-formulated vaccine and normal saline(NS)were used to set up control groups.Virus challenge test was carried out using 10 times the median lethal dose(LD50)of A/Puerto Rico/8/34(H1N1)strain two weeks after the last immunization and the protective effects were assessed through measuring the dynamic changes in body weight and survival rate.Results Higher levels of serum HI,IgG1 and IgG2a antibodies and higher HI antibody conversion rates were induced in the adjuvant groups,especially in the aged mice group,than in the control groups.Nano-emulsion adjuvant improved the immunogenicity of HA and mouse immunity to A/Puerto Rico/8/34(H1N1).Conclusions Nano-emulsion adjuvant could enhance the immunogenicity of influenza antigens,especially in aged mice.

关 键 词：佐剂 流感 纳米乳剂 老龄鼠 免疫原性 

分 类 号：S85[农业科学—兽医学]