我国南方部分地区鸭疫里氏杆菌的分离鉴定及耐药情况调查  

Identification and antibiotic resistance analysis of Riemerella anatipestifer isolated from diseased ducks in south China

作　　者：朱元军 王小莺[1] 杨德鸿 麦凯杰 刘洋洋 罗翠芬 周庆丰 刘军发 罗军荣[1] ZHU Yuanjun;WANG Xiaoying;YANG Dehong;MAI Kaijie;LIU Yangyang;LUO Cuifen;ZHOU Qingfeng;LIU Junfa;LUO Junrong(College of Animal Science and Technology,Jiangxi Agricultural University,Nanchang 330045,China;Research Institute of WENS Foodstuff Group Co.Ltd.,Xinxing 527400,China;College of Animal Sicence and Technology/College of Veterinary Medicine,Huazhong Agricultural University,Wuhan 430070,China)

机构地区：[1]江西农业大学动物科学技术学院,江西南昌330045 [2]温氏食品集团股份有限公司研究院,广东新兴527400 [3]华中农业大学动物科技学院/动物医学院,湖北武汉430070

出　　处：《畜牧与兽医》2019年第12期106-111,共6页Animal Husbandry & Veterinary Medicine

基　　金：广东省扬帆计划引进紧缺拔尖人才项目(2014);广东省科技创新战略专项资金(2018B030315002);温氏食品集团科研基金(ZB20180801CCG002);江西省科技支撑计划重点项目(20151BBF60046);江西省教育厅科技计划项目(GJJ11397)

摘　　要：通过对我国南方地区患疑似浆膜炎的病鸭进行细菌分离纯化,对所得菌株革兰氏染色镜检,PCR鉴定及序列分析,最终分离到120株鸭疫里氏杆菌,并对菌株进行耐药检测。发现菌株对丁胺卡那、卡那霉素、链霉素、四环素、罗红霉素、红霉素、阿莫西林、青霉素、氨苄西林和环丙沙星有很强的耐药性,同时对头孢曲松、头孢吡肟、多西环素、左氟氧沙星和氟苯尼考呈现出较强的敏感性。通过对氨基糖苷类耐药基因的检测,发现在氨基糖苷类耐药菌株中存在大量的耐氨基糖苷类耐药基因。120 strains of Riemerella anatipestifer were isolated from the diseased ducks in south China,and were verified by Gram Staining,PCR and sequence analysis.The antimicrobial susceptibility of these strains was examined.The strains were found to be highly resistant to gentamicin,amikacin,kanamycin,streptomycin,tetracycline,roxithromycin,erythromycin,amoxicillin,penicillin,ampicillin,and ciprofloxacin,and to be strongly sensitive to ceftriaxone,cefepime,doxycycline,levofloxacin and florfenicol.In the aminoglycoside-resistant strains,a large number of aminoglycoside-resistant genes were detected by PCR.

关 键 词：鸭疫里默氏杆菌 抗生素 耐药性 药敏实验 耐药基因 

分 类 号：S855.1[农业科学—临床兽医学]