益气养阴凉血方对免疫性血小板减少性紫癜模型小鼠CD4^+CD25^+调节性T细胞的影响  

Effects of Yiqi Yangyin Liangxue method on CD4^+CD25^+ regulatory T cells in immune thrombocytopenia purpura mice model

作　　者：胡琏 刘松山[1] 赵冰洁[1] 车虹[1] 王译 肖利 王雪梅[2] 赵茜[2] 刘培佳 何娅玲 Hu Lian;Liu Songshan;Zhao Bingjie;Che Hong;Wang Yi;Xiao Li;Wang Xuemei;Zhao Qian;Liu Peijia;He Yaling(Department of Hematopathy,the Affiliated Hospital of Chengdu University of Traditional Chinese Medicine,Chengdu,Sichuan 610072,China;Graduate Student 2016 of Chengdu University of Traditional Chinese Medicine,Chengdu,Sichuan 610072,China)

机构地区：[1]成都中医药大学附属医院血液科,610072 [2]成都中医药大学,610072

出　　处：《中国基层医药》2019年第23期2817-2821,共5页Chinese Journal of Primary Medicine and Pharmacy

基　　金：四川省科技厅应用基础研究基金(2017JY0331)。

摘　　要：目的 观察益气养阴凉血方对免疫性血小板减少性紫癜(ITP)模型小鼠外周血血小板计数、CD4^+CD25^+调节性T细胞(Treg)的影响.方法 将100只小鼠随机分为空白对照组、造模组、激素组、中药组、中药+激素组,每组20只.除空白对照组外,其余各组均通过使用抗小鼠血小板血清(APS)建立ITP模型,于造模开始后第8天,除空白对照组以外其余各组给予相应剂量药物灌胃,连续给药14 d.使用兽用血液分析仪测定造模前、灌胃前、灌胃后各实验组小鼠的血小板计数,灌胃后采用流式细胞仪检测各组小鼠CD4^+CD25^+ Treg.结果 灌胃后各组与造模组比较,中药组、激素组、中药+激素组小鼠外周血血小板计数明显上升[(413.55 ± 38.84)×10^9/L,(710.45 ± 124.52)×10^9/L,(768.10 ± 127.42)×10^9/L,(908.05 ± 89.66)× 10^9/L,t= -10.18、-11.90、-22.63,均 P <0.01],外周血 CD4^+CD25^+ Treg 细胞所占比例均明显上升[(1.649 ± 0.286)%,(2.000 ± 0.193)%,(2.286 ± 0.271)%,(2.648 ± 1.883)%,t = -4.54、-7.221、-13.031,均P<0.01].中药+激素组各指标与中药组和激素组比较差异均有统计学意义(t= -5.759、-4.107、-10.762、-4.902,均 P <0.01).结论 益气养阴凉血方或许可通过上调 ITP 小鼠外周血中CD4^+CD25^+ Treg细胞表达,从而减少血小板过度破坏.Objective To observe the effect of Yiqi Yangyin Liangxue method on peripheral blood platelet count and CD4^+CD25^+ regulatory T cells(Treg)in mice model of immune thrombocytopenic purpura(ITP).Methods A total of 100 mice were randomly divided into blank control group,model group,Chinese medicine group,hormone group,Chinese medicine+hormone group,with 20 mice in each group.In addition to the blank control group,the other four groups were intraperitoneally injected with guinea pig anti -mouse platelet serum(APS)to establish the ITP model.The peripheral blood platelet counts of mice in each group were determined by animal blood analyzer before modeling,before and after gavage,and CD4^+CD25^+ Treg cells in each group were detected by flow cytometry after gavage.Results After intragastric administration,compared with the model group,the peripheral blood platelet count of mice in the other groups increased significantly [(413.55 ± 38.84)×10^9/L,(710.45 ± 124.52)×10^9/L,(768.10 ± 127.42)×10^9/L,(908.05 ± 89.66)×10^9/L,t= -10.18,-11.90,-22.63,all P<0.01],and the proportion of CD4^+CD25^+ Treg cells in peripheral blood increased significantly [(1.649 ± 0.286)%,(2.000 ± 0.193)%,(2.286 ± 0.271)%,(2.648 ± 1.883)%,t= -4.54,-7.221,-13.031,all P<0.01].All indicators of the Chinese medicine+hormone group had statistically significant differences compared with those of the Chinese medicine group and the hormone group(t = -5.759,-4.107,-10.762,-4.902,all P <0.01).Conclusion Yiqi Yangyin Liangxue method may up-regulate the expression of CD4^+CD25^+ Treg cells in peripheral blood of ITP mice,thereby reducing excessive platelet damage.

关 键 词：紫癜 血小板减少性 免疫 中草药 医学 中国传统 糖皮质激素类 T淋巴细胞 小鼠 疗效比较研究 

分 类 号：R28[医药卫生—中药学]