世诺二代CK-MB试剂测定CK-MB的优势  

Advantages of the Second Generation CK-MB Reagent Kit in Detecting Serum CK-MB Activity

作　　者：张莉 沈东华[1] 许中[1] ZHANG Li;SHEN Dong-hua;XU Zhong(Department of Clinical Laboratory,East District of Suzhou Municipal Hospital,Suzhou Hospital Affiliated to Nanjing Medical University,Suzhou,Jiangsu,215001,China)

机构地区：[1]南京医科大学附属苏州医院,苏州市立医院检验科,江苏苏州215001

出　　处：《中国血液流变学杂志》2019年第2期223-225,共3页Chinese Journal of Hemorheology

摘　　要：目的评估世诺二代肌酸激酶同工酶-MB(CK-MB)试剂(CK-S、CKMB-S)抑制CK-MB活性假性升高的性能.方法收集申能德赛CK-MB试剂(CK-D、CKMB-D)筛查异常血清90例,使用CK-S、CKMB-S重测,为实验组;CK-D、CKMB-D复检结果为对照组.采用日立7600全自动生化分析仪检测,均为免疫抑制法.结果CKMB-S/CK-S×100%多分布在0%~30%,CKMB-D/CK-D多分布在>30%的区域.CKMB-D/CK-D、CKMB-S/CK-S倒置率分别为25.6%、4.4%,χ^2=15.730,P<0.01;比例异常率分别为56.7%、14.4%,χ^2=35.011,P<0.01;大于参考区间上限率分别为17.8%、8.9%,χ^2=3.077,P>0.05.结论添加了抗线粒体CK抗体的CK-S、CKMB-S试剂,使CK-MB检测结果更接近于真值.Objective The second generation creatine kinase isoenzyme-MB (CK-MB) reagent (CK-S, CKMB-S) was evaluated for its ability to inhibit the pseudo-increased activity of CK-MB activity. Methods Ninety patients with abnormalities detected by Shenneng Desai CK-MB reagent (CK-D, CKMB-D) were collected and retested by CK-S, CKMB-S for the experimental group;CK-D, CKMB-D result of the retest was for the control group. 7600 automatic biochemical analyzer was used, all with immunosuppression. Results CKMB-S/CK-S ×100% distribution was in 0%~30%, CKMB-D/CK-D distribution in >30%. The conversion rates of CKMB-D/CK-D and CKMB-S/CK-S were 25.6% and 4.4%, respectively, χ^2=15.730, P<0.01;the abnormal rates were 56.7% and 14.4%, respectively, χ^2=35.011, P<0.01;the upper limit ratio is greater than the reference interval of 17.8%, 8.9%, χ^2=3.077, P>0.05. Conclusion The addition of CK-S, CKMB-S reagents of MtCK makes the CK-MB detection result closer to the true value.

关 键 词：肌酸激酶同工酶 线粒体肌酸激酶同工酶 

分 类 号：R446.11[医药卫生—诊断学]