EXPORTIN 1A prevents transgene silencing in Arabidopsis by modulating nucleo-cytoplasmic partitioning of HDA6  

作　　者：Guohui Zhu Yanan Chang Xuezhong Xu Kai Tang Chunxiang Chen Mingguang Lei Jian-Kang Zhu Cheng-Guo Duan 

机构地区：[1]Department of Horticulture and Landscape Architecture,Purdue University,West Lafayett,1N 47907,USA [2]College of Life Sciences,South China Agricultural University,Guangzhou 510642,China [3]Shanghai Center for Plant Stress Biology and Center of Excellence for Molecular Plant Sciences,the Chinese Academy of Sciences,Shanghai 201602,China [4]The University of Chinese Academy of Sciences,Beijing 100049,China

出　　处：《Journal of Integrative Plant Biology》2019年第12期1243-1254,共12页植物学报（英文版）

基　　金：supported by the Strategic Priority Research Program of the Chinese Academy of Sciences(XDB27040203);supported by Chinese Academy of Sciences;supported by the National Natural Science Foundation of China(31570250)

摘　　要：In eukaryotic cells,transport of macromolecules across the nuclear envelope is an essential process that ensures rapid exchange of cellular components,including protein and RNA molecules.Chromatin regulators involved in epigenetic control are among the molecules exported across the nuclear envelope,but the significance of this nucleo-cytoplasmic trafficking is not well understood.Here,we use a forward screen to isolate XPO1 A(a nuclear export receptor in Arabidopsis)as an anti-silencing factor that protects transgenes from transcriptional silencing.Loss-of-function of XPO1 A leads to locus-specific DNA hypermethylation at transgene promoters and some endogenous loci.Wefound that XPO1 A directly interacts with histone deacetylase HDA6 in vivo and that the xpo1 a mutation causes increased nuclear retention of HDA6 protein and results in reduced histone acetylation and enhanced transgene silencing.Our results reveal a new mechanism of epigenetic regulation through the modulation of XPO1 A-dependent nucleo-cytoplasm partitioning of a chromatin regulator.In eukaryotic cells, transport of macromolecules across the nuclear envelope is an essential process that ensures rapid exchange of cellular components,including protein and RNA molecules. Chromatin regulators involved in epigenetic control are among the molecules exported across the nuclear envelope, but the significance of this nucleo-cytoplasmic trafficking is not well understood. Here, we use a forward screen to isolate XPO1 A(a nuclear export receptor in Arabidopsis)as an anti-silencing factor that protects transgenes from transcriptional silencing. Loss-of-function of XPO1 A leads to locus-specific DNA hypermethylation at transgene promoters and some endogenous loci. Wefound that XPO1 A directly interacts with histone deacetylase HDA6 in vivo and that the xpo1 a mutation causes increased nuclear retention of HDA6 protein and results in reduced histone acetylation and enhanced transgene silencing. Our results reveal a new mechanism of epigenetic regulation through the modulation of XPO1 A-dependent nucleo-cytoplasm partitioning of a chromatin regulator.

关 键 词：ENVELOPE RETENTION EXCHANGE 

分 类 号：O57[理学—粒子物理与原子核物理]