Saccharification of Sugarcane Bagasse Using an Enzymatic Extract Produced by Aspergillus fumigatus  

作　　者：Kenia F.R.Lamounier Patrisia O.Rodrigues Daniel Pasquini Milla A.Baffi 

机构地区：[1]Institute of Agricultural Sciences,Federal University of Uberlândia(UFU),Uberlândia,MG,38408-100,Brazil [2]Chemistry Institute,Federal University of Uberlândia(UFU),Uberlândia,MG,38408-100,Brazil

出　　处：《Journal of Renewable Materials》2018年第2期169-175,共7页可再生材料杂志（英文）

摘　　要：This study investigates the efficiency of a crude enzymatic extract produced by Aspergillus fumigatus SCBM6 by solid state fermentation(SSF)in the hydrolysis of alkali pretreated sugarcane bagasse(PTB).After SSF using in natura sugarcane bagasse(SCB),the enzymatic extract presented 21.33 U.g^-1 of β-glucosidase and 544.46 U.g^-1 of xylanase.The alkaline pretreatment with sodium hydroxide 2%NaOH(w/v)removed 43% of the lignin from PTB and the cellulosic fraction increased to 75%.The hydrolysis was optimized as a function of time,temperature,and concentration of PTB.After hydrolysis,the maximum yield(30.05%)of total released reducing sugars(TRS)was obtained under the following conditions:24 h,55℃,2% of PTB and 3 U.g^-1 of β-glucosidase(CBU).Furthermore,an approximate TRS value(26.4%)was also obtained after saccharification carried out during 6 h,55℃,4% of PTB and 1 CBU.These results indicate that hydrolysis can be performed in a short incubation period and with low enzymatic load for reasonable TRS release.

关 键 词：A.fumigatus enzymatic hydrolysis sugarcane bagasse alkaline pretreatment solid state fermentation 

分 类 号：O61[理学—无机化学]