美国白蛾乙酰胆碱受体基因的筛选与鉴定  

Screening and Identification of Nicotinic Acetylcholine Receptors in Hyphantria cunea

作　　者：武建业 郝锡联[1] 李嘉新 周宇鑫 王寅亮[2] Wu Jiangye;Hao Xilian;Li Jiaxin;Zhou Yuxin;Wang Yinliang(Ji-lin Normal University,Siping 136000,P.R.China;Northeast Normal University)

机构地区：[1]吉林师范大学,吉林省四平市136000 [2]东北师范大学

出　　处：《东北林业大学学报》2019年第12期95-99,共5页Journal of Northeast Forestry University

基　　金：吉林省科技发展计划-重点科技攻关项目(20160204023NY)

摘　　要：乙酰胆碱受体一般在生物体内存在两个类型--烟碱型乙酰胆碱受体(Nicotinic acetylcholine receptors,nAChRs)和毒蕈型乙酰胆碱受体(Muscarinic acetylcholine receptors,mAChRs),均为重要的功能型膜蛋白。nAChRs是cys-loop配体门控离子通道超家族的成员,介导昆虫中枢神经系统突触胆碱能的快速运输,是一些新烟碱类等杀虫剂的重要靶点;而mAChRs是离子通道蛋白G蛋白偶联受体的超家族。试验以美国白蛾为研究对象,基因组测序分析获得12个乙酰胆碱受体,并对其进行一级结构以及结构域进行预测、同源性分析、实时荧光定量PCR等。结果表明:可将其分类为10种nAChR和2种mAChR,并且各AChR在不同鳞翅目中相对保守;测定不同组织AChR表达量中发现,AChR多表达于头部、胸部,部分位于触角、腹部。Acetylcholine receptors in organisms commonly exists in two forms-Nicotinic acetylcholine receptors(nAChRs)and Muscarinic acetylcholine receptors(mAChRs),are the important functional membrane proteins.nAChRs,a member of the cys-loop ligand-gated ion channel superfamily,mediates the rapid transport of choline energy in the synapses of the central nervous system of insects,and is an important target for some neonicotinoids and other pesticides.MAChRs is a superfamily of G protein-coupled receptors of ion channel proteins.With the fall webworm as the research object,genome sequencing analysis of 12 acetylcholine receptors,and carries on the primary structure and predict structure domain,homology analysis,real-time fluorescent quantitative PCR,such as research,the results show that it can be classified as 10 kinds of nAChR and 2 kinds of mAChR,and each AChR is relatively conservative in different lepidoptera;when different organizational AChR expression was measured,AChR was mostly found in the head and chest,partly in tentacles and abdomen.

关 键 词：美国白蛾 乙酰胆碱受体 生物信息学分析 实时荧光定量PCR 

分 类 号：S763.42[农业科学—森林保护学] Q786[农业科学—林学]