功能电刺激对脊髓损伤大鼠肢体运动功能及损伤脊髓ERK表达的影响  

Effect of functional electrical stimulation on limb motor function and extra cellular signal-regulated kinase expression in adult rats with spinal cord injury

作　　者：王维 郭跃 王文娟 蔡洪梅 Wang Wei;Guo Yue;Wang Wenjuan;Cai Hongmei(Department of Rehabilitation,First Affiliated Hospital of Jinzhou Medical University,Jinzhou 121000,China)

机构地区：[1]锦州医科大学附属第一医院康复医学科,锦州121000

出　　处：《中华神经医学杂志》2019年第12期1224-1228,共5页Chinese Journal of Neuromedicine

基　　金：国家自然科学基金(81601767)。

摘　　要：目的探讨功能电刺激对脊髓损伤大鼠肢体运动功能的保护作用及机制。方法健康雌性SD大鼠120只按随机数字表法分为假手术组(40只)、模型组(40只)、功能电刺激组(40只)。后2组大鼠采用NYU打击器制作脊髓损伤模型,假手术组大鼠只作椎板切除术,不进行脊髓损伤,功能电刺激组大鼠于损伤后8 h开始进行功能电刺激治疗。分别在脊髓损伤后l d、1周、2周、4周每组取10只大鼠,用BBB评分评定大鼠的运动功能,免疫组化染色检测损伤脊髓细胞外信号调节激酶(ERK)的表达,实时定量PCR(RT-PCR)检测损伤脊髓ERK mRNA的表达。结果与假手术组比较,模型组及功能电刺激组大鼠伤后各时间点BBB评分降低,损伤脊髓ERK蛋白、ERK mRNA的表达均增高,差异有统计学意义(P<0.05);脊髓损伤后1周、2周、4周功能电刺激组大鼠BBB评分高于模型组,ERK蛋白、ERK mRNA的表达低于模型组,差异有统计学意义(P<0.05)。结论功能电刺激可通过抑制损伤脊髓ERK的表达,对脊髓起到一定的保护作用,从而达到改善脊髓损伤大鼠肢体运动功能的效果。Objective To investigate the protective effect of functional electrical stimulation on limb motor function and its mechanism in rats with spinal cord injury.Methods One hundred and twenty healthy adult SD rats were randomly divided into sham-operated group,model group,and functional electrical stimulation(FES)group(n=40).The rats in the latter two groups were replicated as spinal cord injury models on T9 using NYU method;and only laminectomy without spinal cord injury was performed in rats of the sham-operated group;FES was performed in rats of the functional electrical stimulation group 8 h after model making.One d,and one,two,and four weeks after model making,10 rats in each group were chosen for experiment,respectively;BBB scale was used to evaluate the motor function of the rats;immunohistochemical staining was used to detect the extra cellular signal-regulated kinase(ERK)expression in the injured spinal cords;ERK mRNA expression in the injured spinal cords was detected by real-time PCR.Results As compared with rats in the control group,rats in the model group and FES group had significantly decreased BBB scale scores and statistically increased ERK protein and mRNA expressions at each time point after injury(P<0.05).One,two,and four weeks after model making,rats in the FES group had significantly higher BBB scale scores and statistically lower ERK protein and mRNA expressions than rats in the model group(P<0.05).Conclusion FES can protect the spinal cords by inhibiting the ERK expression in the injured areas,thereby improving the limb motor function of rats with spinal cord injury.

关 键 词：功能电刺激 脊髓损伤 细胞外信号调节激酶 BBB评分 

分 类 号：R651[医药卫生—外科学]