无鱼粉日粮条件下黄颡鱼生长性能和肝胰脏转录组差异表达的研究  

Fishmeal-free diet can reduce the growth performance and the hepatopancreas transcriptional significant differential expression of yellow catfish, Pelteobagrus fulvidraco

作　　者：周露阳 叶元土[1] 蔡春芳[1] 吴萍[1] 高敏敏 郁浓 孙飞 吕昊 石瑶瑶 吕斌 易皓明 ZHOU Lu-yang;YE Yuan-tu;CAI Chun-fang;WU Ping;GAO Min-min;Yu Nong;SUN Fei;LV Hao;SHI Yao-yao;LV Bin;YI Hao-ming(School of Basic Medical Sciences and Biotechnology,Soochow University,Suzhou 215123,China)

机构地区：[1]苏州大学基础医学与生物科学学院

出　　处：《海洋科学》2019年第12期97-109,共13页Marine Sciences

基　　金：校企合作项目(P113410618)~~

摘　　要：本研究以黄颡鱼(Pelteobagrus fulvidraco)为试验对象,设计两种饲料:鱼粉组和无鱼粉组,在池塘网箱中投喂70 d,研究其生长性能和肝胰脏转录组表达的差异。同鱼粉组相比,无鱼粉组黄颡鱼特定生长率下降了30.25%(P<0.05),饲料系数显著增加了83.94%(P<0.05),全鱼脂肪含量显著下降(P<0.05),血清甘油三酯含量显著下降(P<0.05),血清转氨酶活力显著增加(P<0.05)。肝胰脏转录组结果显示,总计有12020个基因差异表达,其中差异表达上调的基因有5020个、差异表达下调的基因有7000个。同鱼粉组相比,无鱼粉组分别有1013个基因表达显著上调、2749基因表达显著下调(P<0.05)。将组间具有差异表达的基因进行GO Term、KEGG pathway分类,结果显示:无鱼粉组的肝胰脏细胞组成、细胞生物过程、细胞分子功能等绝大多数基因差异表达下调,提示肝胰脏的细胞组织结构和功能受到很大的影响;KEGG通路富集到15个显著差异代谢通路。本研究结果表明,日粮中鱼粉可能含有某些生理活性物质,这些物质以神经分泌、激素调控、代谢信号通路等为作用靶点,通过对这些代谢信号通路的调节,对鱼的整体生理代谢强度、细胞结构与功能、生理健康状态等产生明显积极促进影响。缺乏这些物质则会造成黄颡鱼生长性能下降、部分器官组织细胞(肝细胞、神经轴突)受到损伤、鱼体抗应激能力和免疫防御能力下降。In the present study,the effects of dietary fish meal on the growth performance and physiological me-tabolism of yellow catfish,Pelteobagrus fulvidraco,were investigated.We prepared two diets containing 28%Pe-ruvian fish meal(control diet,FM)and fishmeal-free(test diet,NF),which were isonitrogenous,isoenergetic,and isophosphate.Diets were offered to yellow catfish reared in net cages(12.02±0.11 g)for 70 days.Special grow rate(SGR)decreased by 30.25%(P<0.05),feed coefficient rate(FCR)increased to 83.94%(P<0.05),and crude lipid content significantly decreased in the fish fed the NF diet compared with those fed the FM diet(P<0.05).In addition,the concentration of triglycerides in their serum significantly decreased(P<0.05)and the activity of se-rum aminotransferase significantly increased in fish fed the NF diet(P<0.05).Hepatopancreas transcriptome an-notation showed that in total,12020 genes were differentially expressed,of which 5020 were upregulated and 7000 were downregulated.In total,1013 genes were significantly upregulated and 2749 genes were downregulated in the NF diet compared with the FM diet(P<0.05).The GO term and KEGG pathway were used to classify dif-ferentially expressed genes between the two diets.Results showed that most of the differentially expressed genes were downregulated in the cell composition,cell biological processes,and cell molecular function in the hepato-pancreas of fish fed the NF diet,suggesting that the cellular tissue structure and function of their hepatopancreas were affected to a certain extent.Fifteen considerably different metabolic pathways were enriched by KEGG,in-cluding axons,neurohormone secretion,hormone secretion and regulation,metabolic signaling pathway,inflam-matory factor transmission,anti-stress,immune defense,and other important pathways.These results indicated that fish meal may contain some physiologically active substances.These physiologically active substances can affect neurosecretory,hormone regulation,metabolic signal pathway,and other processes and have

关 键 词：鱼粉 生长 信号通路 神经 激素 黄颡鱼(Pelteobagrus fulvidraco) 

分 类 号：S965.199[农业科学—水产养殖]