2016-2018年钦州市手足口病住院患儿病原学检测结果分析  

The pathogenic composition of hospitalized children with hand,foot and mouth disease in Qinzhou during 2016-2018

作　　者：鲍金圭 李崇建[1] 江慧 柳明波[1] 李仕娟[1] 梁玲 BAO Jingui;LI Chongjian;JIANG Hui;LIU Mingbo;LI Shijuan;LIANG Ling(Department of Clinical Laboratory,The First People's Hospital of Qinzhou,Qinzhou,Guangxi 535000,China;Department of Scientific Research,The First People's Hospital of Qinzhou,Qinzhou,Guangxi 535000,China)

机构地区：[1]广西壮族自治区钦州市第一人民医院检验科,广西钦州535000 [2]广西壮族自治区钦州市第一人民医院科研科,广西钦州535000

出　　处：《检验医学与临床》2019年第24期3592-3595,共4页Laboratory Medicine and Clinic

基　　金：广西壮族自治区钦州市科研项目（201616810）

摘　　要：目的分析2016-2018年该院住院患儿手足口病的病原构成及其变化,为钦州地区儿童手足口病的防治提供科学依据。方法采集2016-2018年该院诊断为手足口病的住院患儿粪便标本778份,采用实时荧光定量PCR方法,进行肠道病毒通用型(EV)、肠道病毒71型(EV71)、柯萨奇病毒A组16型(CA16)、CA6和CA10的检测。分析不同性别、不同年龄及不同月份肠道病毒型的检出率是否存在差异。结果2016-2018年各年收治的患儿数分别为266、319、193例,病原检测阳性率分别为88.72%、89.34%、89.12%。其中2016年以EV71型为主,2017年以CA6为主,2018年以CA16为主。各型肠道病毒阳性患儿的构成比在不同年份之间比较,差异均有统计学意义(P<0.05)。患儿主要为4岁以下儿童,1~<2岁儿童高发。手足口病发病高峰期在5-7月以及9-10月。手足口病感染率每年从4月份开始上升,11月份下降,呈现夏秋季节流行特征。结论2016-2018年钦州市手足口病优势病原体存在一定差异,分别为EV71、CA6及CA16型。持续检测病原体的变化对手足口病的防治具有重要的临床意义。Objective To explore pathogenic composition of hand,foot and mouth disease(HFMD)in the first people′s hospital of Qinzhou during 2016-2018,to provide the scientific basis for its treatment and control.Methods A total of 778 samples of HFMD cases which collected from the hospital from 2016 to 2018 were tested for enterovirus(EV),EV71,coxsackie virus(CA)16,CA6 and CA10 by real-time reverse transcription polymerase chain reaction(RT-PCR),and the differences of EV types among different genders,ages and months were analyzed.Results There were 266 cases in 2016,319 cases in 2017 and 193 cases in 2018.And the positive rates were 88.72%,89.34% and 89.12%,respectively.The EV71,CA6 and CA16 type were the main type from 2016 to 2018.The proportion of the number of EV positive cases were compared between different years,and the difference was statistically significant(P<0.05).The HFMD mainly occurred in children under four years old,especially in children aged 1-2 years old in Qinzhou.The peak of HFMD was from May to July and September to October in Qinzhou.Infection rate increased from April and decreased from November,which was popular in summer and autumn.Conclusion The dominant pathogens of HFMD are different in Qinzhou from 2016 to 2018,which is EV71,CA6 and CA16 type.Therefore,the detection of the etiology of HFMD is extremely important for its prevention and treatment.

关 键 词：手足口病 肠道病毒 实时荧光定量PCR 柯萨奇病毒 

分 类 号：R446.5[医药卫生—诊断学]