Sheng Jiang San, traditional multi-herb formulation, exerts anti-influenza effects in vitro and in vivo via neuraminidaseinhibition and immune regulation  

作　　者：ZHANG Tian-bo XIAO Meng-jie Chun-Kwok WONG Ka-Pun CHRIS MOK ZHAO Xin TI Hui-hui Pang-Chui SHAW 

机构地区：[1]State Key Laboratory of Respiratory Disease,Guangdong Provincial Key Laboratory of Molecular Target&Clinical Pharmacology,School of Pharmaceutical Sciences and The Fifth Affiliated Hospital,Guangzhou Medical University,Guangzhou,China [2]The School of Life Sciences,The Chinese University of Hong Kong,Hong Kong,China [3]School of Public Health,Li Ka Shing Faculty of Medicine,The University of Hong Kong,Hong Kong,China

出　　处：《中国药理学与毒理学杂志》2019年第9期738-739,共2页Chinese Journal of Pharmacology and Toxicology

摘　　要：OBJECTIVE Sheng Jiang San(SJS),a multi-herb formulation,is used in treating high fever,thirsty and anxiety in ancient China and it is sometimes used to treat seasonal influenza in modern.However,there is no evidencebased investigation and mechanism research to support SJS′s anti-influenza efficacy.This study aims to investigate the anti-influenza effect of SJS and its possible mechanisms.METHODS In this study,we examined the inhibitory effect of SJS against different influenza viruses on Madin-Darby canine kidney cells.Influenza virus infected BALB/c mice were employed as in vivo model to evaluate the efficacy.Mice challenged with A/PR/8/34(H1N1)were orally administrated SJS 1 g·kg^-1 daily for seven days and monitored for 14 d.The survival rate,body mass changes,lung index,lung viral load,histopathologic changes and immune-regulation of the mice were measured.The underlying anti-influenza virus mechanisms were studied by a series of biological assays in vitro to determine if hemagglutinin,ribonucleoprotein complex or nerauminidase were targets of SJS.RESULTS SJS exerted a broad spectrum of inhibitory effects on multiple influenza strains in a dose-dependent manner.And IC50 of SJS against A/WSN/33(H1N1)was lower than 35 mg·L^-1.SJS also protected 50%of mice from influenza virus PR8 infection.The lung index and the lung viral load of SJS treated mice were signifi⁃cantly decrease compared with untreated mice.SJS 2 g·L^-1 inhibited 80%of neuraminidase enzymatic activity.SJS also up-regulated TNF-αand IFN-αand down-regulated IL-2 of influenza virus induced mice.CONCLUSION SJS is a useful formulation for treating influenza virus infection.OBJECTIVE Sheng Jiang San（SJS）, a multi-herb formulation, is used in treating high fever, thirsty and anxiety in ancient China and it is sometimes used to treat seasonal influenza in modern. However, there is no evidencebased investigation and mechanism research to support SJS′s anti-influenza efficacy. This study aims to investigate the anti-influenza effect of SJS and its possible mechanisms. METHODS In this study, we examined the inhibitory effect of SJS against different influenza viruses on Madin-Darby canine kidney cells. Influenza virus infected BALB/c mice were employed as in vivo model to evaluate the efficacy. Mice challenged with A/PR/8/34（H1 N1） were orally administrated SJS 1 g·kg-1 daily for seven days and monitored for 14 d. The survival rate, body mass changes, lung index, lung viral load, histopathologic changes and immune-regulation of the mice were measured. The underlying anti-influenza virus mechanisms were studied by a series of biological assays in vitro to determine if hemagglutinin, ribonucleoprotein complex or nerauminidase were targets of SJS. RESULTS SJS exerted a broad spectrum of inhibitory effects on multiple influenza strains in a dose-dependent manner. And IC50 of SJS against A/WSN/33（H1 N1） was lower than 35 mg·L-1. SJS also protected50% of mice from influenza virus PR8 infection. The lung index and the lung viral load of SJS treated mice were significantly decrease compared with untreated mice. SJS 2 g·L-1 inhibited 80% of neuraminidase enzymatic activity. SJS also up-regulated TNF-α and IFN-α and down-regulated IL-2 of influenza virus induced mice. CONCLUSION SJS is a useful formulation for treating influenza virus infection.

关 键 词：Sheng Jiang San anti-influenza activity neuraminidase inhibition immuno-regulation 

分 类 号：R75[医药卫生—皮肤病学与性病学]