TGF-β1对兔膝后交叉韧带损伤后LOXs表达的影响  

Effect of TGF-β1 on the expression of LOXs in the posterior cruciate ligament injury of rabbits

作　　者：陈荣富 符纯锋 王春莉[3] 陈诚[4] CHEN Rongfu;FU Chun-feng;WANG Chun-li;CHEN Cheng(The Second Department of Orthopaedics,People's Hospital of Changshou,Chongqing 401220,China;The First Department of Orthopaedics,Dazhou Integrated TCM&Western Medicine Hospital,Dazhou 635000,China;College of Bioengineering,Chongqing Universityy Chongqing 400044,China;Department of Orthopedics,The First Affiliated Hospital,Chongqing Medical Universityt Chongqing 400016,China)

机构地区：[1]重庆市长寿区人民医院骨二科,重庆401220 [2]四川省达州市中西医结合医院骨一科,635000 [3]重庆大学生物工程学院,重庆400044 [4]重庆医科大学附属第一医院骨科,重庆400016

出　　处：《中国矫形外科杂志》2019年第23期2174-2178,共5页Orthopedic Journal of China

基　　金：重庆市卫生和计划生育委员会医学科研项目（编号：2016MSXM155）

摘　　要：[目的]探讨TGF-β1对兔膝后交叉韧带损伤后LOXs表达的影响。[方法]选取36只成年新西兰大白兔,按照实验方法分成PCL损伤组、PCL损伤+TGF-β1组和假手术组,每组各12只。分别于术后的0、7、14、21d处死3只白兔,取后交叉韧带组织,应用RT-PCR检测各时间三组白兔LOXs家族的基因表达量。[结果]术后0 d三组白兔LOXs家族的基因表达量差异无统计学意义(P>0.05),但在术后7、14、21 d PCL损伤组LOXs家族的基因表达量低于假手术组(P<0.05);PCL损伤+TGF-β1组LOXs家族的基因表达量高于相应时间点PCL损伤组(P<0.05)。[结论]TGF-β1可能通过调节LOXs及其家族的表达参与PCL的愈合过程。[Objective]To investigate the effect of TGF-β1 on the expression of LOXs after injury of the posterior cruciate ligament.[Methods]Thirty-six adult New Zealand white rabbits were selected and divided into the PCL injury group,PCL injury+TGF-β1 group and sham group according to the experimental treatment,with 12 animals in each group.Three rabbits were sacrificed at 0 d,7 d,14 d and 21 d after operation in each group.The posterior cruciate ligament tissue was used to detect the gene expression of LOXs family in three groups of rabbits at different time points by RTFQ PCR.[Results]There was no significant difference in the expression of LOXs family between the three groups at 0 day(P>0.05).However,the expression of LOXs in the PCL injury group was significantly lower than that in the sham group at 7 days,14 days and 21 days after surgery(P<0.05).The expression of LOXs in PCL injury+TGF-β1 group was significantly higher than that in the PCL injury group at the corresponding time point(P<0.05).[Conclusion]TGF-β1 might play an important role in the healing process of PCL injury by regulating the expression of LOXs.

关 键 词：后交叉韧带损伤 赖氨酰氧化酶 转化生长因子-Β1 

分 类 号：R318[医药卫生—生物医学工程]