新疆维吾尔自治区蜱中布鲁菌的分子流行病学分析  

Molecular epidemiology analysis of Brucella in ticks in Xinjiang Uygur Autonomous Region

作　　者：姜蒙蒙 杨梅花[3] 宋瑞霞 王远志[4] 盛金良 Jiang Mengmeng;Yang Meihua;Song Ruixia;Wang Yuamhi;Sheng Jinliang(Department of Basic Veterinary Medical Sciences,School of Animal Science and Technology,Shihezi University,Shihezi 832002,China;Xinjiang Uygur Autonomous Regional Center for Disease Control and Prevention,Urumqi 830011,China;Department of Forestry,School of Agriculture,Shihezi University,Shihezi 832000,China;Department of Pathology Biology and Immunology,School of Medicine,Shihezi University,Shihezi 832000,China)

机构地区：[1]新疆石河子大学动物科技学院基础兽医学教研室,832002 [2]新疆维吾尔自治区疾病预防控制中心,乌鲁木齐830011 [3]新疆石河子大学农学院林学系,832000 [4]新疆石河子大学医学院病原生物学与免疫学教研室,832000

出　　处：《中华地方病学杂志》2019年第12期947-950,共4页Chinese Journal of Endemiology

基　　金：国家自然科学基金(81560338);“十三五”国家传染病重大专项(2018ZX10101002-002-007)。

摘　　要：目的掌握新疆维吾尔自治区(简称新疆)蜱中布鲁菌分子流行病学特征,为制定有效的防控措施提供科学依据.方法2016-2018年,在新疆边境10个县(市)采集家畜体表寄生蜱(包括孕雌蜱),在阿拉山口市采用布旗法采集游离蜱.将孕雌蜱分别放入透气昆虫管中进行产卵,每管卵堆平均分成两部分:一部分为蜱卵;另一部分继续发育,直至为幼蜱为止.对所有蜱类进行分子生物学(16S rRNA)鉴定,确定其蜱种.提取蜱DNA,基于布鲁菌外膜蛋白omp22和IS711两个基因进行PCR检测,将扩增产物测序并进行BLAST比对分析.结果在新疆的11个县(市)共采集1084只蜱,其中寄生蜱747只(包括孕雌蜱34只),游离蜱337只.经16S rRNA基因鉴定,确定为4属5种,草原革蜱、边缘革蜱、刻点血蜱、亚洲璃眼蜱、图兰扇头蜱所占比例分别为29.43%(319/1084)、16.51%(179/1084)、10.42%(113/1084)、37.27%(404/1084)、6.37%(69/1084).共检出214份布鲁菌阳性核酸样本,阳性率为19.74%.寄生蜱阳性率为25.30%(189/747),游离蜱阳性率为7.42%(25/337),寄生蜱阳性率明显高于游离蜱(χ2=46.873,P<0.05).在34份"孕雌蜱-蜱卵"发育阶段检出2份羊种布鲁菌核酸,在22份"蜱卵-幼蜱"发育阶段检出1份羊种布鲁菌核酸.结论布鲁菌在新疆边境地区寄生蜱和游离蜱中广泛分布,寄生蜱阳性率较高.羊种布鲁菌在蜱中存在潜在的经卵和经期传播现象,在家畜布鲁菌病的防控过程中,应加强体表寄生蜱和环境中游离蜱的灭蜱工作.Objective To carry out a investigation on molecular epidemiological features of tick-borne Brucella in Xinjiang Uygur Autonomous Region(Xinjiang),and to provide a scientific basis for formulation of effective preventive and control measures.Methods In 2016-2018,parasitic ticks(including engorged females)were collected on the body surface of livestock in 10 counties(cities)along the border of Xinjiang.The free-living ticks were collected by flagging method in Alashankou.The engorged female was placed in a breathable insect tube for spawning,each egg batch was divided into two parts:one part was tick eggs,while the second part was allowed further larval development.All ticks were identified by molecular biology(16S rRNA)identification.Tick DNA was extracted,PCR was performed based on Brucella omp22 and IS711,and amplification products were sequenced and analyzed by BLAST.Results A total of 1084 ticks were collected in 11 counties(cities),of them 747 were parasitic ticks(including 34 engorged females)and 337 were free-living ticks.Based on 16S rRNA identification,1084 ticks belonged to 4 genera and 5 species,and the proportions of Dermacentor nuttalli,Dermacentor marginatus,Haemaphysalis punctata,Hyalomma asiaticum and Rhipicephalus turanicus were 29.43%(319/1084),16.51%(179/1084),10.42%(113/1084),37.27%(404/1084),and 6.37%(69/1084),respectively.A total of 214 Brucella-positive nucleic acid samples were detected,the positive rate was 19.74%.The parasitic ticks'positive rate was 25.30%(189/747),and the free-living ticks'positive rate was 7.42%(25/337),parasitic ticks'positive rate was higher than that of free-living ticks(χ2=46.873,P<0.05).Two Brucella melitensis nucleic acid samples were detected in 34"engorged females-tick eggs"developmental stage,and one Brucella melitensis nucleic acid sample was detected in 22"tick eggs-larvae"developmental stage.Conclusions Brucella is widely distributed in parasitic ticks and free-living ticks in Xinjiang border areas,and the parasitic ticks'positive rate is obviously higher

关 键 词：蜱 布鲁杆菌属 经卵传播 经期传播 

分 类 号：S85[农业科学—兽医学]