组织染色法观察灰盖鬼伞子实体发育过程（英文）  

Tissue Stainingto Studythe FruitingProcess of Coprinopsis cinerea

作　　者：SUBBA Shanta WINKLER Marco KüES Ursula SUBBA Shanta;WINKLER Marco;KüES Ursula(Molecular Wood Biotechnology and Technical Mycology Institute and Goettingen Center for Molecular Biosciences(GZMB),University of Goettingen,Biisgenweg,Goettingen 37077,Germany)

机构地区：[1]树木分子生物技术与菌物技术研究院哥廷根分子生物科学中心(GZMB)哥廷根大学

出　　处：《食用菌学报》2019年第4期29-38,共10页Acta Edulis Fungi

摘　　要：灰盖鬼伞(Coprinopsis cinerea)是一种嗜粪模式食用菌,可在实验室条件下快速生长并结实。子实体发育从黑暗环境下形成初级菌丝结开始,随后在光照刺激下初级菌丝结紧密聚集形成二级菌丝结,然后二级菌丝结继续分化出菌柄和菌盖。在子实体形成培养条件下,前5天(P1-P5)为原基发育期,第6天担子及担孢子中发生核融合及减数分裂,伴随菌柄伸长和菌盖形成,子实体发育成熟。成熟的子实体在第7天自溶,含有孢子的液滴落地并释放出孢子。本文运用不同的组织染色技术观察原基组织切片,从而发现灰盖鬼伞子实体发育过程中不同的组织和细胞类型。前担子比其他组织更容易被乳酚蓝、迈尔士苏木精明矾、孔雀绿、曙红染色。菌盖皮最容易被孔雀绿和乳酚蓝染色。过碘酸雪夫氏染色(periodic acid Schiff,PAS)的菌盖皮和子实基层颜色比其他组织深,其中菌盖皮颜色很深,子实基层颜色稍深。所有切片还尝试了各种可能组合的双染。在PAS和苏木精明矾先后双染色下,子实基层和前胆子可以获得最佳区分。The coprophilous Coprinopsis cinereais an edible model mushroom that grows and fruits very fast under laboratory conditions.Fruiting starts with primary hyphal knot(Pk)formation in the dark,followed by light-induced compact aggregates known as the secondary hyphal knots(Sks)in which stipe and cap tissues differentiate.Under defined fruiting conditions,primordium development(P1 to P5)takes five days to culminate on Day 6 of development in karyogamy(K)and meiosis(M)within the basidia and subsequent basidiospore production which parallels fruiting body maturation(stipe elongation and cap expansion).Mature fruiting bodies autolyze on Day 7 to release the spores in liquid droplets to the ground.By applying different histochemical staining techniques for the light microscopy,primordial sections of C.cinerea were examined to study different tissues and cell types which appear over the time in the fruiting process.Lactophenol blue,Mayer’s hemalum,malachite green and eosin all stained probasidia stronger than other tissues.The pileipellis was best stained by malachite green and lactophenol blue.Periodic acid Schiff(PAS)stained the pileipellis better dark and the subhymenium slightly darker as compared to other tissues.All stains were also applied in combinations of two,in both possible orders.In double staining with PAS and hemalum,the subhymenium and probasidia were best differentiated.

关 键 词：子实体 组织染色 子实基层 担子 糖原 灰盖鬼伞 

分 类 号：S64[农业科学—蔬菜学]