三种植物精油对草菇害虫双额岩小粪蝇的熏杀击倒及其混配增效作用  

Fumigation Effect of Three Plant Essential Oils Against Bifronsina bifrons(Stenhammar) and Their Synergistic Effect

作　　者：吴华 戴建青 陈大嵩 欧剑峰 黄鸿 WU Hua;DAI Jianqing;CHEN Dasong;OU Jianfeng;HUANG Hong(Guangdong Key Laboratory of Animal Conservation and Resource Utilization,Guangdong Public Laboratory of Wild Animal Conservation and Utilization,Guangdong Insttute of Applied Biological Resources,Guangzhou,Guangdong510260,China)

机构地区：[1]广东省生物资源应用研究所广东省动物保护与资源利用重点实验室广东省野生动物保护与利用公共实验室

出　　处：《食用菌学报》2019年第4期137-142,共6页Acta Edulis Fungi

基　　金：广州市科技计划项目(201607010105);广东省科学院科技发展专项(2017GDASCX-0107)

摘　　要：双额岩小粪蝇(Bifronsina bifrons)是广州地区草菇(Volvariella volvacea)的主要害虫,本研究采用三角瓶密闭熏蒸法测试月桂烯、α-蒎烯、异松油烯3种植物精油对双额岩小粪蝇的熏杀、击倒及其混配增效作用。三种植物精油对双额岩小粪蝇的24 h熏杀半致死浓度(LC50)为月桂烯(5.05μL/L)>α-蒎烯(29.92μL/L)>异松油烯(38.01μL/L)。在熏蒸浓度均为18μL/L时,月桂烯对双额岩小粪蝇的击倒效果最好,半数击倒时间(KT50)为14.37 min,其次为α-蒎烯、异松油烯,KT50值分别为53.60 min和64.03 min。将月桂烯与α-蒎烯以体积比1∶9、3∶7、5∶5、7∶3和9∶1混配后具有增效作用,最佳混配比是5∶5;实验中最高熏蒸浓度的月桂烯、α-蒎烯和异松油烯对草菇菌丝生长无显著影响。本研究为草菇生产中双额岩小粪蝇的无公害防治及高效环保的植物源灭蝇剂的开发提供参考。The fly Bifronsina bifrons(Stenhammar)is the major insect pest that infects Volvariella volvacea in Guangdong.Using the fumigation in closed bottle method,insecticidal activity and fumigation knockdown effect of plant essential oils from myrcene,α-pinene,and terpinolene and their mixtures in combinations of two against B.bifrons were tested.The results showed that the three kinds of plant essential oils possessed high fumigation insecticidal activity and fumigation knockdown effect against B.bifrons.The LC50 values for essential oils from myrcene,α-pinene,and terpinolene against B.bifrons after 24 h fumigation were 5.05μL/L,29.92μL/L and 38.01μL/L,respectively.When the fumigation concentration was 18μL/L,the half-knockdown time(KT50)of essential oils from myrcene,α-pinene,and terpinolene against B.bifrons were 14.37 min,53.60 min,and 64.03 min,respectively.The co-toxicity coefficient of myrcene andα-pinene mixtures at volumetric ratios of 3:7,5:5 and 7:3 against B.bifrons were 245.15,344.71,and 237.47,respectively.The mixture of myrcene andα-pinene at 5:5(V/V)showed the best synergistic effect.Mixtures of myrcene andα-pinene with volumetic ratios of 1:9 and 9:1 also showed a stable synergistic effect,and their coefficients of co-toxicity were 189.36 and 177.56,respectively.Mycelial growth of V.volvacea was not affected under the highest fumigation concentration of all three plant essential oils.Therefore,these plant essential oils had a potential to be developed into effective and eco-friendly insecticides.

关 键 词：月桂烯 Α-蒎烯 异松油烯 双额岩小粪蝇 半致死浓度 半数击倒时间 

分 类 号：S64[农业科学—蔬菜学]