青蒿素抑制人肝癌细胞Huh7和SMMC-7721增殖及其机制  

Inhibitory effect and its mechanism of artemisinin on the proliferation of hepatoma Huh7 and SMMC-7721 cells

作　　者：王惠国[1] 李雨桐 蔡珂 韩鑫龙 袁立霞[2] 谭晓梅[2,3] 唐玲 WANG Huiguo;LI Yutong;CAI Ke;HAN Xinlong;YUAN lixia;TAN Xiaomei;TANG Ling(College of Life Science and Technology,Dalian University,Dalian,Liaoning,116622 P.R.China;College of Traditional Chinese Medicine,Southern Medical University,Guangzhou,Guangdong,510515 P.R.China;Guangdong Provincial Key Laboratory of Chinese Medicine Pharmaceutics&Guangdong Provincial Engineering Laboratory of Chinese Medicine Preparation Technology,Southern Medical University,Guangzhou,Guangdong,510515 P.R.China)

机构地区：[1]大连大学生命科学与技术学院,辽宁大连116622 [2]南方医科大学中医药学院,广东广州510515 [3]南方医科大学广东省中药制剂技术工程实验室广东省中药制剂重点实验室,广东广州510515

出　　处：《华西药学杂志》2019年第6期583-586,共4页West China Journal of Pharmaceutical Sciences

基　　金：国家自然科学基金资助项目(批准号:81573661);广东省自然科学基金资助项目(编号:2016A030313621)

摘　　要：目的探讨青蒿素抑制肝癌细胞Huh7和SMMC-7721增殖的作用及其机制。方法不同浓度的青蒿素与人肝癌细胞Huh7和SMMC-7721共培养24、48、72 h后,采用Cell viability法检测细胞的增殖活性;细胞克隆实验检测细胞集落的抑制情况;流式细胞仪检测细胞的凋亡情况;Western Blot法检测细胞内蛋白水平的变化。结果Cell viability与细胞克隆实验的结果表明:与空白对照比较,青蒿素能够增加肝癌细胞Huh7和SMMC-7721的凋亡率。青蒿素通过抑制mTOR信号通路,进而抑制肝癌细胞Huh7和SMMC-7721的增殖。结论青蒿素能够抑制肝癌细胞Huh7和SMMC-7721的增殖,诱导其凋亡,且通过抑制mTOR信号通路活化抑制肝癌细胞Huh7和SMMC-7721的增殖,进而发挥抗肿瘤作用。OBJECTIVE To study the inhibitory effect and its mechanism of artemisinin on the proliferation of Huh7 and SMMC-7721 cells.METHODS Different concentrations of artemisinin were co-cultured with human hepatoma Huh7 and SMMC-7721 cells for 24 h,48 h and 72 h.Cell proliferation activity was measured using Cell viability assay.The inhibition,and apoptosis of cells was detected by Cell cloning assay and flow cytometry,respectively.The changes in intracellular protein levels were detected by Western Blot assay.RESULTS The results of Cell viability assay and Cell cloning assay showed that artemisinin could increase the apoptosis rate of Huh7 and SMMC-7721 cells compared with the control group.Artemisinin inhibits the proliferation of HCC cells Huh7 and SMMC-7721 by inhibiting the mTOR signaling pathway.CONCLUSION Artemisinin can inhibit the proliferation and induce apoptosis of Huh7 and SMMC-7721,and inhibit the proliferation of Huh7 and SMMC-7721 by inhibiting the activation of mTOR signaling pathway,thus exerting the anti-tumor effect.

关 键 词：青蒿素 肝癌 HUH7 SMMC-7721 增殖 凋亡 信号通路 抗肿瘤 细胞活力 蛋白质免疫印迹法 

分 类 号：R96[医药卫生—药理学]