甘草黄酮类化学成分研究  

Studies of flavonoids from Glycyrrhizae Radix et Rhizoma

作　　者：胡耿 黄绮韵 张甜 梅玉丹 唐曦阳 孟鹤 于洋 戴毅[1] 姚新生[1] HU Geng;HUANG Qi-yun;ZHANG Tian;MEI Yu-dan;TANG Xi-yang;MENG He;YU Yang;DAI Yi;YAO Xin-sheng(Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research,College of Pharmacy,Jinan University,Guangzhou 510632,China;Xiangxue Academician Workstation,Xiangxue Pharmaceutical Co.,Ltd.,Guangzhou 510663,China)

机构地区：[1]暨南大学药学院广东省中药药效物质基础和创新药物研究重点实验室,广东广州510632 [2]广州市香雪制药股份有限公司广东省香雪中药及天然药物院士工作站,广东广州510663

出　　处：《中草药》2019年第21期5187-5192,共6页Chinese Traditional and Herbal Drugs

基　　金：广东省科技计划项目(2015B030301005)

摘　　要：目的研究甘草Glycyrrhizae Radix et Rhizoma黄酮类化学成分。方法采用HP-20大孔树脂、硅胶、ODS、凝胶等多种柱色谱,结合制备液相等方法进行分离和纯化,根据理化性质及波谱数据对化学成分进行结构鉴定。结果从甘草70%乙醇提取物中分离得到10个黄酮类化合物,分别鉴定为4′,6,7-三羟基-2′-甲氧基查耳酮(1)、3′,4′,5,7-四羟基-8-(3-羟基-3-甲基丁基)-异黄酮(2)、异甘草素(3)、异甘草苷(4)、刺甘草查耳酮(5)香豌豆酚(6)、芒柄花苷(7)、2(S)-3′,5′,7-三羟基二氢黄酮(8)、南酸枣苷(9)、4′,7-二羟基黄酮(10)。结论化合物1和2为新化合物,分别命名为异甘草查耳酮B和甘草异黄酮G,化合物9为首次从该属植物中分离得到。Objective To study the chemical constituents of flavonoids from Glycyrrhizae Radix et Rhizoma.Methods The compounds were isolated and purified by column chromatography over HP-20 macroporous resin,silica gel,Sephadex LH-20,and preparative RP-HPLC.Their structures were elucidated by physicochemical properties and spectral analyses.Results Ten flavonoids were isolated and identified as 4′,6,7-trihydroxy-2′-methoxyl-chalcone(1),3′,4′,5,7-tetrahydroxy-8-(3-hydroxy-3-methylbutyl)-isoflavone(2),isoliquiritigenin(3),isoliquiritin(4),echinatin(5),orobol(6),ononin(7),2(S)-3′,5′,7-trihydroxyflavanone(8),2(S)-naringenin-4′-O-β-D-glucopyranoside(9),and 4′,7-dihydroxyflavone(10).Conclusion Compounds 1 and 2 are new compounds named isolicochalcone B and licoisoflavone G,while compound 9 is isolated from the genus for the first time.

关 键 词：甘草 黄酮 异甘草查耳酮B 甘草异黄酮G 异甘草苷 刺甘草查耳酮 香豌豆酚 芒柄花苷 南酸枣苷 

分 类 号：R284.1[医药卫生—中药学]