高压环境对BV2小胶质细胞TNF-α和IL-1β表达的影响  

High pressure increases the express of TNF-αand IL-1βin BV2 microglia

作　　者：杨帆 李旭声 李娅 刘小瑜 刘梦婷 丁洪光 曾红科 Yang Fan;Li Xusheng;Li Ya;Liu Xiaoyu;Liu Mengting;Ding Hongguang;Zeng Hongke(Guangdong Provincial People's Hospital,Affiliated to South China University of Technology,Guangzhou 510080,China)

机构地区：[1]华南理工大学附属广东省人民医院,广州510080

出　　处：《中华急诊医学杂志》2019年第12期1507-1510,共4页Chinese Journal of Emergency Medicine

基　　金：广东省自然科学基金(2016A030311043,2017A030313691);广州市科技计划项目(A2019135)。

摘　　要：目的探究高压力对BV2小胶质细胞促炎因子TNF-α和IL-1β表达的影响。方法使用自制加压装置干预体外培养的BV2小胶质细胞。根据干预压力随机分组包括:对照组、20 mmHg组、25 mmHg组、30 mmHg组和35 mmHg组;根据干预时间随机分组包括:对照组、6 h组、12 h组、24 h组。用免疫荧光和WesternBlot方法检测TNF-α和IL-1β蛋白表达变化。结果30 mmHg的压力处理BV2小胶质细胞24 h后,TNF-α和IL-1β的表达明显高于对照组(均P<0.01)、6 h组(均P<0.01)和12 h组(TNF-α:P<0.05;IL-1β:P<0.01)。30 mmHg组TNF-α和IL-1β的表达水平明显高于对照组(均P<0.01)、20 mmHg组(均P<0.01)、25 mmHg组(TNF-α:P<0.05;IL-1β:P<0.01)。30 mmHg组与35 mmHg组比较,TNF-α和IL-1β的表达差异无统计学意义(均P>0.05)。结论高压力可明显升高BV2小胶质细胞TNF-α和IL-1β的表达。Objective This study aimed to explore whether high pressure would increase expression of TNF-a and IL-1β.Methods BV2 microglia cells were treated with a self-made device.BV2 microglia cells were randomly divided into five groups according to different pressures:control group,20 mmHg group,25 mmHg group,30 mmHg group,and 35 mmHg group.BV2 microglia cells were randomly divided into five groups according to different intervention time:control group,6 h group,12 h group,24 h group.TNF-αand IL-1βexpression were assessed by Western Blotting or double immunofluorescence.Results The 30 mmHg group had the highest expression levels of TNF-αand IL-1βas compared with control group(both P<0.01),6 h group(both P<0.01)、12 h group(TNF-α:P<0.05;IL-1β:P<0.01).30 mmHg group had the highest expression levels of TNF-αand IL-1βas compared with control group(bothP<0.01),20 mmHg group(both P<0.01),and 25 mmHg group(TNF-α:P<0.05;IL-1β:P<0.01).The expression levels of TNF-αand L-1βwere not different between 30 mmHg group and 35 mmHg group(both P>0.05).Conclusions High pressure may increase the expression levels of TNF-αand IL-1βof microglia.

关 键 词：压力 小胶质细胞 肿瘤坏死因子-Α 白介素-1Β 

分 类 号：R54[医药卫生—心血管疾病]