黔产景天三七的化学成分及药理活性研究  

Chemical constituents and pharmacological activities of Sedum aizoon form Guizhou province

作　　者：熊燕 杜彩霞[1,2,3] 段玉书 苑春茂 黄烈军 顾玮[1,2] 郝小江 XIONG Yan;DU Cai-xia;DUAN Yu-shu;YUAN Chun-mao;HUANG Lie-jun;GU Wei;HAO Xiao-jiang(State Key Laboratory of Functions and Applications of Medicinal Plants,Guiyang 550014,China;The Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences,Guiyang 550014,China;Pharmacy of Guizhou Medical University,Guiyang 550025,China;State Key Laboratory of Phytochemistry and Plant Resources in West China,Kunming institute of Botany,Chinese Academy of Sciences,Kunming 650201,China)

机构地区：[1]贵州医科大学省部共建药用植物功效与利用国家重点实验室,贵州贵阳550014 [2]贵州省中国科学院天然产物重点实验室,贵州贵阳550014 [3]贵州医科大学药学院,贵州贵阳550025 [4]中国科学院昆明植物研究所植物化学与西部植物资源持续利用国家重点实验室,云南昆明650201

出　　处：《中草药》2019年第22期5404-5410,共7页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(31600280);国家自然科学基金资助项目(U1812403);贵州省科技计划项目(黔科合支撑[2018] 2799号);黔科合平台人才(2017-5737);省部共建药用植物功效与利用国家重点实验室开放课题项目(FAMP201704K)

摘　　要：目的对黔产景天三七Sedum aizoon的化学成分以及抗氧化活性和α-葡萄糖苷酶抑制活性进行研究。方法采用多种色谱技术进行分离纯化,根据EI-MS、ESI-MS、1H-和13C-NMR等波谱数据并结合化合物理化性质进行结构鉴定。利用DPPH法筛选所得化合物的抗氧化活性;用PNPG法对所得化合物进行α-葡萄糖苷酶抑制活性的检测。结果从景天三七的醋酸乙酯部位分离、鉴定得到17个化合物,分别为鸢尾酚酮(1)、山柰酚(2)、槲皮素(3)、杨梅素(4)、鼠李素-3-O-β-D-吡喃葡萄糖(5)、异落叶松脂素-9-O-β-D-吡喃葡萄糖苷(6)、myricitrime(7)、杨梅素-3-O-α-L-阿拉伯糖苷(8)、鸢尾酚酮-2-O-β-D-吡喃葡萄糖(9)、2-O-(trans-caffeoyl)-malic acid 1-methyl ester(10)、2-O-(trans-caffeoyl)-malic acid 1,4-dimethyl ester(11)、2-O-(trans-caffeoyl)malic acid(12)、对香豆酸(13)、没食子酸乙酯(14)、丁二酸(15)、油酸酰胺(16)、百脉根苷(17)。结论化合物13、15为首次从景天三七中分离得到,化合物9~12、16为首次从景天属植物中分离得到。化合物2、3、7、8、10、12、14具有良好的抗氧化活性。化合物8有微弱的体外抑制α-葡萄糖苷酶活性,化合物3有较强的体外抑制α-葡萄糖苷酶活性。Objective To study the chemical constituents of Sedum aizoon and to screen the anti-oxidant activities andα-glucosidase inhibitory activities of compounds.Methods The compounds were separated and purified by various chromatographic techniques,and their structures were identified by physio-chemical properties and EI-MS,ESI-MS,1H and 13C NMR.The anti-oxidant activity of compounds was screened by DPPH method.The obtained compounds were subjected to detection ofα-glucosidase inhibitory activity by PNPG method.Results Seventeen compounds were isolated from S.aizoon,which were identified as iriflophene(1),kaempferol(2),quercetin(3),myricetin(4),rhamnazin-3-O-β-D-glucopyranoside(5),isolariciresinol-9-O-β-D-glucopyranoside(6),myricitrime (7), myricetin-3-O-α-L-arabinopyranoside (8), iriflophenone-2-O-β-D-glucopyranoside (9), 2-O-(trans-caffeoyl)-malicacid 1-methyl-ester (10), 2-O-(trans-caffeoyl)-malic acid 1,4-dimethyl ester (11), 2-O-(trans-caffeoyl) malic acid (12), p-coumaric acid(13), ethyl gallate (14), butanedioic acid (15), 9(Z)-octadece-namide (16), and lotaustralin (17). Conclusion Compounds 13 and 15are isolated from S. aizoon for the first time. Compounds 9—12, and 16 are isolated from genus Sedum for the first time. Compounds 2,3, 7, 8, 10, 12, and 14 had significant anti-oxidant activity. Compounds 8 showed moderate α-glucosidase inhibitory activity in vitro.Compound 3 showed strong α-glucosidase inhibitory activity in vitro.

关 键 词：景天三七 槲皮素 杨梅素-3-O-α-L-阿拉伯糖苷 鸢尾酚酮-2-O-β-D-吡喃葡萄糖 没食子酸乙酯 油酸酰胺 

分 类 号：R284.1[医药卫生—中药学]