荷包牡丹碱对人肝癌MHCC97-H细胞转移、侵袭的影响及机制研究  

Effects of dicentrine on migration and invasion of human hepatocellular carcinoma MHCC97-H cells and potential mechanisms

作　　者：郭锰[1] 张成辉[1] 吴婷婷[1] GUO Meng;ZHANG Cheng-hui;WU Ting-ting(Department of Oncology,Nanyang Central Hospital,Nanyang 473009,China)

机构地区：[1]南阳市中心医院肿瘤内科

出　　处：《中草药》2019年第22期5515-5520,共6页Chinese Traditional and Herbal Drugs

摘　　要：目的探讨荷包牡丹碱对人肝癌MHCC97-H细胞转移、侵袭的影响及潜在机制。方法体外培养MHCC97-H细胞至对数生长期,分别经5、10、25、50、100μmol/L荷包牡丹碱处理24、48、72 h,MTT法检测细胞增殖能力。5、10、25μmol/L荷包牡丹碱处理MHCC97-H细胞24 h,MTT法检测细胞黏附能力,细胞划痕实验检测细胞转移能力,Transwell小室实验检测细胞侵袭能力,实时荧光定量PCR(qRT-PCR)实验检测VEGF、MMP-2及MMP-9基因表达水平,蛋白免疫印迹实验检测p-JAK2及p-STAT3蛋白表达水平。结果25μmol/L荷包牡丹碱处理MHCC97-H细胞48、72 h及50、100μmol/L荷包牡丹碱处理24、48、72 h后的细胞活力下降明显,与对照组比较,差异显著(P<0.05、0.01);5、10、25μmol/L荷包牡丹碱处理MHCC97-H细胞24 h,细胞黏附能力、伤口愈合能力、穿膜数均明显下降(P<0.05、0.01),VEGF、MMP-2、MMP-9基因表达及p-JAK2、p-STAT3蛋白表达均明显下降(P<0.05、0.01)。结论荷包牡丹碱可以抑制人肝癌MHCC97-H细胞的转移及侵袭,该作用与下调VEGF、MMP-2、MMP-9基因表达及抑制JAK2/STAT3信号通路的活化有关。Objective To detect the effects of dicentrine on the migration and invasion of human hepatocellular carcinoma MHCC97-H cells and potential mechanisms.Methods MHCC97-H cells were cultured in vitro and cultured at logarithmic growth stage.The cells were treated with 5,10,25,50,100μmol/L dicentrine for 24,48,72 h,and cell proliferation was determined by MTT assay.The cells were treated with 5,10,25μmol/L dicentrine for 24 h,the cell adhesion,cell migration,cell invasion,gene expression of VEGF,MMP-2 and MMP-9,and protein expression of p-JAK2 and p-STAT3 were determined by MTT,scratch,Transwell,real-time qPCR,and western blot assay,respectively.Results The cell viability of MHCC-97H cells treated with 25μmol/L dicentrine at 48 and 72 h,and 50 and 100μmol/L dicentrine at 24,48,and 72 h was decreased significantly,which was significantly different from that of the control group(P<0.05 or P<0.01).Compared with control group,cell adhesion ability,wound healing ability,cell penetration modulus,gene expression of VEGF,MMP-2 and MMP-9,and protein expression of p-JAK2 and p-STAT3 of MHCC-97H cells treated with 5,10 and 25μmol/L dicentrine at 24 h were decreased significantly(P<0.05 or P<0.01).Conclusion Dicentrine can inhibit the migration and invasion of human hepatocellular carcinoma MHCC97-H cells,which is related to the down-regulation of VEGF,MMP-2,and MMP-9 gene expression and inhibition of JAK2/STAT3 signaling pathway activation.

关 键 词：荷包牡丹碱 人肝癌MHCC97-H细胞 转移 侵袭 JAK2/STAT3信号通路 

分 类 号：R285.5[医药卫生—中药学]