A Molecular Framework for the Control of Adventitious Rooting by TIR1/AFB2-Aux/IAADependent Auxin Signaling in Arabidopsis  

作　　者：Abdellah Lakehal Salma Chaabouni Emilie Cavel Rozenn Le Hir Alok Ranjan Zahra Raneshan Ondrej Novak Daniel IPacurar Irene Perrone Frangois Jobert Laurent Gutierrez Laszlo Bako Catherine Bellini 

机构地区：[1]Umea Plant Science Centre,Department of Plant Physiology,Umea University,90736 Umea,Sweden [2]lnstitut Jean-Pierre Bourgin,INRA,AgroParisTech,CNRS,Universite Paris-Saclay,78000 Versailles,France [3]Department of Biology,Faculty of Science,Shahid Bahonar University,Kerman,Iran [4]Laboratory of Growth Regulators,Faculty of Science,Palacky University and Institute of Experimental Botany,The Czech Academy of Sciences,78371 Olomouc,Czech Republic [5]Umea Plant Science Centre,Department of Forest Genetics and Physiology,Swedish Agriculture University,90183 Umea,Sweden [6]Centre de Ressources Regionales en Biologie Moleculaire(CRRBM),Universite de Picardie Jules Verne,80039 Amiens,France [7]Present address:Centre de Ressources Regionales en Biologie Moleculaire(CRRBM),Universite de Picardie Jules Verne,80039 Amiens,France [8]Present address:Institute for Sustainable Plant Protection,National Research Council of Italy,Turin,Italy [9]Present address:Umea Plant Science Center,Department of Forest Genetics and Plant Physiology,SLU,90736 Umea,Sweden

出　　处：《Molecular Plant》2019年第11期1499-1514,共16页分子植物（英文版）

摘　　要：In Arabidopsis thaliana,canonical auxin-dependent gene regulation is mediated by 23 transcription factors from the AUXIN RESPONSE FACTOR(ARF)family that interact with auxin/indole acetic acid repressors(Aux/IAAs),which themselves form co-receptor complexes with one of six TRANSPORT INHIBITOR*!/AUXIN-SIGNALLING F-BOX(TIR1/AFB)proteins.Different combinations of co-receptors drive specific sensing outputs,allowing auxin to control a myriad of processes.ARF6 and ARF8 are positive regulators of adventitious root initiation upstream of jasmonate,but the exact auxin co-receptor complexes controlling the transcriptional activity of these proteins has remained unknown.Here,using loss-of-function mutants we show that three Aux/IAA genes,IAA6,IAA9,and IAA17,act additively in the control of adventitious root(AR)initiation.These three IAA proteins interact with ARF6 and/or ARF8 and likely repress their activity in AR development.We show that TIR1 and AFB2 are positive regulators of AR formation and TIR1 plays a dual role in the control of jasmonic acid(JA)biosynthesis and conjugation,as several JA biosynthesis genes are up-regulated in the tir1-1 mutant.These results lead us to propose that in the presence of auxin,TIR1 and AFB2 form specific sensing complexes with IAA6,IAA9,and/or IAA17 to modulate JA homeostasis and control AR initiation.

关 键 词：TIR1/AFB AuxIAA JASMONATE adventitious roots ARABIDOPSIS 

分 类 号：Q94[生物学—植物学]