紫外诱变酵母菌利用餐饮废水油脂制取生物柴油  

Biodiesel Production by UV Mutagenesis Yeast Feeding Catering Wastewater

作　　者：柴宝华 王怡[1,2,3] 王文怀 范攀 CHAI Bao-hua;WANG Yi;WANG Wen-huai;FAN Pan(School of Environmental and Municipal Engineering,Xi'an University of Architecture and Technology,Xi'an 710055,China;Shaanxi Key Laboratory of Environmental Engineering,Xi'an University of Architecture and Technology,Xi'an 710055,China;Northwest China Key Laboratory of Water Resources and Environment Ecology,Xi'an University of Architecture and Technology,Xi'an 710055,China)

机构地区：[1]西安建筑科技大学环境与市政工程学院,陕西西安710055 [2]西安建筑科技大学陕西省环境工程重点实验室,陕西西安710055 [3]西安建筑科技大学西北水资源与环境生态教育部重点实验室,陕西西安710055

出　　处：《中国给水排水》2019年第21期113-117,共5页China Water & Wastewater

基　　金：陕西省自然科学基础研究重点项目(2016JZ019)

摘　　要：基于解脂耶氏酵母菌能够利用疏水基质作为碳源在胞内积累油脂的特点,通过紫外诱变提高其脂质积累能力,并将其用于模拟餐饮含油废水的处理,最终将积累的酵母菌用于生物柴油的制取。试验结果表明,在照射功率为15 W、波长为257.3 nm的紫外诱变条件下,通过照射不同的时间,获得了高产油酵母菌YL13,将其在产油培养基中培养7 d后的生物量浓度、脂质产量、生物柴油产量分别为未诱变菌的1.43、1.66和1.79倍。将诱变培养的YL13用于含20 g/L油脂的模拟餐饮废水处理时,7 d后对油脂的去除率达到71%,且生物柴油的产量达到2.01 g/L。可见,通过紫外诱变的解脂耶氏酵母菌可以利用餐饮废水中的油脂,在降低废水中油脂浓度的同时能够通过酵母菌的脂质积累制取生物柴油。Lipid accumulation ability of Yarrowia lipolytica(YL)was enhanced by ultraviolet mutagenesis based on characteristics that YL could utilize hydrophobic substrates as the carbon source to accumulate intracellular lipids.The mutagenic YL was then used to treat a simulated catering wastewater and accumulate the lipid for biodiesel production.The results showed that a high-yielding yeast—YL13 was selected after different ultraviolet irradiation time with irradiation power of 15 W and wavelength of257.3 nm.After being cultured in oil-producing medium for 7 days,the biomass concentration,lipid yield and biodiesel production of YL13 were 1.43 times,1.66 times and 1.79 times of that of the unmutated yeast,respectively.Moreover,the oil utilization rate reached 71%and the biodiesel yield was 2.01 g/L when YL13 was used for treating simulated catering wastewater with an oil concentration of 20 g/L.The YL mutagenized by ultraviolet radiation could effectively utilize and reduce the oil from the catering wastewater.Meanwhile,the accumulated lipid by the yeast could be used to produce biodiesel.

关 键 词：解脂耶氏酵母菌 紫外诱变 脂质积累 生物柴油 餐饮废水 

分 类 号：TU992[建筑科学—市政工程]