聚赖氨酸在基因治疗技术中的研究进展  

Research Progress in Application of Poly(L-lysine) in Gene Therapy

作　　者：李从欣 王雪[1] 张晴[1] 贺广锋 耿艳辉[1] LI Congxin;WANG Xue;ZHANG Qing;HE Guangfeng;GENG Yanhui(State Key Laboratory of Antibody Research and Development,New Drug Research and Development Co.,Ltd.,North China Pharmaceutical Corporation,Shijiazhuang 050015,China)

机构地区：[1]华北制药集团新药研究开发有限责任公司抗体药物研制国家重点实验室

出　　处：《化学与生物工程》2019年第12期1-4,28,共5页Chemistry & Bioengineering

基　　金：国家科技重大专项重大新药创制专项(2017ZX09303008)

摘　　要：随着生物技术的高速发展,基因治疗受到越来越多的关注。基因治疗在遗传病以及癌症等疾病治疗方面具有很多优势。但是由于裸DNA分子存在性质不稳定、与细胞膜相容性差、易被核酸酶降解等弊端,迫切需要开发高效安全、组织特异性的基因载体材料。聚赖氨酸是一种天然的阳离子多肽,具有良好的生物相容性和生物可降解性,能有效压缩DNA形成复合物纳米粒子。主要概述了聚赖氨酸作为非病毒基因载体在基因治疗中的研究进展,阐述了聚赖氨酸作为非病毒基因载体的重要意义,探讨了聚赖氨酸作为非病毒基因载体在携带DNA传递到靶细胞过程中存在的问题及解决方法。With the rapid development of biotechnology,gene therapy has attracted more and more attention.Gene therapy has many advantages in the treatment of hereditary diseases and cancer.However,due to the unstable property of naked DNA molecules,poor compatibility with cell membranes,and easy degradation by nucleases,it is imperative to develop efficient,safe,and tissue-specific gene carrier materials.Poly(L-lysine)is a natural cationic polypeptide,which has good biocompatibility and biodegradability,and can effectively compress DNA to form complex nanoparticles.Therefore,we summarize the progress in researches of poly(L-lysine)as a non-viral gene carrier in gene therapy,review the significance of poly(L-lysine)as a non-viral gene carrier,and discuss the problems and solutions of poly(L-lysine)as a non-viral gene carrier in carrying DNA to target cells.

关 键 词：聚赖氨酸 基因治疗 线状聚赖氨酸 树枝状聚赖氨酸 

分 类 号：TQ464[化学工程—制药化工]