高粱不同类型胚性和非胚性愈伤组织的生理生化差异  

Physiological and biochemical differences in different kinds of embryogenic and non-embryogenic calluses of Sorghum bicolor

作　　者：季艳丽 程云伟 陈发菊 张德春 周超 JI Yanli;CHENG Yunwei;CHEN Faju;ZHANG Dechun;ZHOU Chao(Biotechnology Research Center,China Three Gorges University,Yichang 443002,Hubei,China)

机构地区：[1]三峡大学生物技术研究中心

出　　处：《广西植物》2019年第12期1613-1618,共6页Guihaia

基　　金：国家自然科学基金(31371596)~~

摘　　要：植物体细胞胚胎发生过程中伴随着复杂的生理生化变化,为进一步揭示胚性愈伤组织的再生潜力,该研究以高粱Sb19未成熟胚诱导产生的两种胚性愈伤组织和一种非胚性愈伤组织为材料,通过测定各愈伤组织中可溶性蛋白、游离脯氨酸和可溶性糖的含量,采用方差分析法对高粱体细胞胚胎发生过程中不同类型愈伤组织的生理生化指标进行了差异比较研究。结果表明:(1)高粱两种胚性愈伤组织中可溶性蛋白、游离脯氨酸和可溶性糖的含量均显著高于非胚性愈伤组织,表明胚性愈伤组织中的代谢活性高于非胚性愈伤组织,能够为体细胞胚胎发生提供更多的物质能量基础。(2)两种类型胚性愈伤组织之间生理生化差异显著,其中,Ⅱ型胚性愈伤组织中可溶性蛋白和游离脯氨酸含量均显著高于I型胚性愈伤组织,相反,Ⅱ型胚性愈伤组织中可溶性糖含量显著低于I型胚性愈伤组织,这种生理生化差异在一定程度上影响了后期的分化。该研究结果为愈伤组织的胚胎发生能力与生化代谢的关系提供理论依据。There are complex physiological and biochemical changes in the process of plant somatic embryogenesis.In order to reveal the regenerative potential of embryogenic callus,we determined the content of soluble protein,free proline and soluble sugar in two kinds of embryogenic calluses and a non-embryonic callus which were from the induction of Sb19 Sorghum bicolor immature embryos.The differences of physiological and biochemical parameters between different types of callus in S.bicolor somatic embryogenesis were compared by variance analysis.The results were as follows:(1)The contents of soluble protein,free proline and soluble sugar in two kinds of embryogenic callus were significantly higher than that of non-embryogenic callus,which indicates that the metabolic activity in embryogenic callus is higher than that in non-embryonic callus,and embryogenic callus can provide more material energy basis for somatic embryogenesis.(2)The physiological and biochemical differences between the two types of embryogenic callus were also significant.Among them,the contents of soluble protein and free proline in TypeⅡembryogenic callus were significantly higher than that of Type I embryogenic callus.In contrast,the content of soluble sugar in TypeⅡembryogenic callus was significantly lower than that of Type I embryogenic callus.We draw a conclusion that the differences of physiological and biochemical affect the differentiation on a certain degree.This study provides a theoretical basis for the relationship between embryogenic ability and biochemical metabolism of callus.

关 键 词：高粱 胚性愈伤组织 非胚性愈伤组织 可溶性蛋白 游离脯氨酸 可溶性糖 

分 类 号：Q945.6[生物学—植物学]