石蛙皮中胶原蛋白提取工艺研究  

Study on the Extraction Technology of Collagen in the Skin of Rana Spinosa

作　　者：蓝松[1] 查道远 Lan Song;Zha Daoyuan(College of Chemistry and Bioengineering of Yichun University,Yichun336000;16 Graduates of Yichun University,Yichun 336000,China)

机构地区：[1]宜春学院化学与生物工程学院

出　　处：《广东化工》2019年第24期20-21,共2页Guangdong Chemical Industry

摘　　要：目的:以石蛙皮为实验主材料,提取胶原蛋白。方法:应用酸酶结合的方法,采用单因素实验筛选最佳胶原蛋白提取工艺。结果:经过筛选,提取工艺为:胃蛋白酶加入量为 3.0 ‰;酶解温度为 25 ℃;酶解时间为 24 h;料液比为 1∶20。结论:该工艺稳定可行,可用于石蛙皮中胶原蛋白的提取。Objective: use skin of Rana spinosa as experimental material, to extract collagen. Methods: the single factor experiment screening the best extraction technology of collagen, application combining with acid and enzyme methods from Wa skin extracted collagen. Results: after screening, extraction process: the adding amount of pepsin was 3.0 per thousand;enzymolysis temperature of 25℃;enzymatic hydrolysis time was 24h;solid liquid ratio of 1∶20. Conclusion: the process is stable and feasible. Can be used for the extraction of collagen in the skin of Rana spinosa.

关 键 词：石蛙皮 胶原蛋白 

分 类 号：TQ[化学工程]