一种猪瘟兔化弱毒活疫苗效力检验方法的研究  

Study on a Method for Detecting the Titer of Live Attenuated Swine Fever Vaccine

作　　者：郑朝朝[1,2] 刘云涛 李倬[3] 邹立宏 郁宏伟 ZHENG Zhao-zhao;LIU Yun-tao;LI Zhuo;ZOU Li-hong;YU Hong-wei(Ringpu(Baoding)Biological Pharmaceutical Co.Lid.,Baoding,Hebei 071000,China;Hebei Engineering and Technology Research Center of Veterinary Biological Products,Baoding,Hebei 071000,China;Jilin Entry-Exit Inspection and Qurantine Bureau,Changchun 130062,China)

机构地区：[1]瑞普(保定)生物药业有限公司,河北保定071000 [2]河北省兽医生物技术工程技术研究中心,河北保定071000 [3]吉林省进出口检验检疫局,长春130062

出　　处：《中国兽药杂志》2019年第12期7-11,共5页Chinese Journal of Veterinary Drug

基　　金：河北省科技计划项目(16226609D)。

摘　　要：为克服兔体定型热反应法(RID)检验猪瘟活疫苗效力的过程中存在的缺陷,建立了一种快速、准确、稳定检测猪瘟病毒含量及疫苗效力的间接免疫荧光法(IFA).该方法以出现特异性荧光作为检测标准,通过添加促细胞感染剂聚乙二醇10000(PB)增加特异性荧光数量,可以在4 d内检测出猪瘟活疫苗成品或半成品效力.该方法检验周期短、结果准确、重复性好,为猪瘟活疫苗效力检验方法的完善提供了数据支持.In order to overcome the defects of the existing methods for testing the effectiveness of classical swine fever vaccine,a rapid and stable method for detecting the titer of classical swine fever vaccine was established.In this method,specific fluorescence was used as the standard of detection,and the specific fluorescent quantity was increased by adding PB,and titer of swine fever live vaccine could be tested in 4 days.The method established in this study can meet the needs of batch detection,and provide a reference for the further improvement of the detection method.

关 键 词：间接免疫荧光 猪瘟活疫苗 效力检验 

分 类 号：S852.65[农业科学—基础兽医学]