刀豆球蛋白A诱导的葡聚糖纳米凝胶高层级自组装  

High Order Self-assembly of Dextran Nanogels Induced by Con A

作　　者：徐源 刘灵珊 王灏[1] 窦红静[1] XU Yuan;LIU Lingshan;WANG Hao;DOU Hongjing(State Key Laboratory of Metal Matrix Composites,School of Materials Science and Engineering,Shanghai Jiao Tong University,Shanghai 200240,China)

机构地区：[1]上海交通大学材料科学与工程学院金属基复合材料国家重点实验室

出　　处：《功能高分子学报》2020年第1期30-38,共9页Journal of Functional Polymers

基　　金：国家自然科学基金（21871180）;上海市“科技创新行动计划”基础研究领域项目（18JC1413500）;上海高校特聘教授（东方学者）计划（SHDP201802）;上海市教委“曙光计划”（12SG12）资助

摘　　要：基于葡聚糖这类结构明确的水溶性多糖,以接枝聚合诱导自组装法(GISA)制备得到具有交联结构的葡聚糖纳米凝胶。在此基础上,利用刀豆球蛋白A(Con A)与葡聚糖中葡萄糖单元之间的特异性识别作用,诱导葡聚糖纳米凝胶的高层级自组装,从而制备尺度可控的Con A-葡聚糖纳米凝胶高层级自组装体。通过透射电镜、红外光谱、核磁共振光谱、等温滴定量热法等手段对高层级自组装体的粒径、结构和形貌进行表征,探讨了其高层级自组装的机理。此外,还探究了Con A以及Con A-葡聚糖纳米凝胶高层级自组装体对人肺癌细胞A549的细胞毒性。结果表明:该自组装体的尺寸同葡聚糖与Con A的质量比直接相关;游离的Con A对A549细胞具有抑制作用,且Con A在参与高层级自组装的过程中生物活性保持不变。A dextran nanogel(Dex NG)with cross-linked structure was prepared through a grafting copolymerization induced self-assembly(GISA)strategy using water-soluble polysaccharide dextran as the main component.GISA is a method that combines free radical grafting polymerization and self-assembly into one step,and can realize large-scale preparation of nanogels.By using cerium ammonium nitrate(CAN)to create free radicals on dextran,methyl acrylate(MA)was initiated to polymerize at the site of free radical to form a grafting copolymer,and a subsequent formation of nanogel was induced by hydrophobic force originated from the resulting MA.Finally,diallyl disulfide(DADS)was added as a cross-linker to stablize the structures.The high order self-assembled nano-aggregates(Con A-Dex NG)were fabricated by Dex NG and concanavalin A(Con A)on the basis of the specific recognition between Con A and the glucose unit in dextran.The particle size,structure and morphology of the self-assembled nano-aggregates were characterized by transmission electron microscopy(TEM),dynamic laser light scattering(DLS),Fourier transform infrared spectroscopy(FT-IR),nuclear magnetic resonance spectroscopy(NMR)and isothermal titration calorimetry(ITC).The mechanism of high order self-assembly was also discussed.In addition,the cytotoxicity of free Con A and Con A-Dex NG on A549 cell(human lung cancer cell)was investigated.The results showed that the size of Con A-dextran nano-aggregates was directly related to the mass ratio of dextran nanogel to Con A.Moreover,the cytotoxicity experiments demonstrated that free Con A could inhibit A549 cells,and its biological activity did not show obvious variations during the process of high order self-assembly.

关 键 词：葡聚糖 刀豆球蛋白A 纳米凝胶 高层级自组装 凝集素-多糖相互作用 

分 类 号：O636.1[理学—高分子化学]