不同外源激素在诱导愈伤过程中对油棕叶片褐化率的影响  

Different Exogenous Hormones in the Process of Callus Induction: Effects on the Browning Rate of Oil Palm Leaf

作　　者：张大鹏[1,2] 王永 石鹏[1,2] 金龙飞 赵志浩[1,2] 曹红星 Zhang Dapeng;Wang Yong;Shi Peng;Jin Longfei;Zhao Zhihao;Cao Hongxing(Coconut Research Institute,CATAS,Wenchang Hainan 571339;Hainan Key Laboratory of Tropical Oil Crops Biology,Wenchang Hainan 571339)

机构地区：[1]中国热带农业科学院椰子研究所,海南文昌571339 [2]海南省热带油料作物生物学重点实验室,海南文昌571339

出　　处：《中国农学通报》2019年第33期47-51,共5页Chinese Agricultural Science Bulletin

基　　金：中国热带农业科学院基本科研业务费专项资金(1630152017009);中央财政林业科技推广示范资金(琼[2016]TG07号);农业部物种资源保护(农作物)项目(2016NWB052)

摘　　要：为了研究不同种类植物激素在油棕成熟叶片组织培养过程中对外植体褐化率的影响。本试验以油棕成熟叶片为诱导材料,以2,4-D、NAA、IBA、IAA、KT、TDZ、6-BA为不同激素处理,分析在其诱导下油棕叶片褐化率。结果表明:(1)6-BA和IBA处理下叶片褐化率最高,分别达到66.98%和65.35%,显著高于其他5种处理;(2)KT处理的叶片褐化率低于6-BA、IBA和TDZ,达到极显著差异的水平;(3)2,4-D、NAA、IAA对叶片的褐化率影响在各个水平上均无显著差异。建议可以在愈伤组织诱导率相近的条件下应优先考虑使用KT,以上结果可以为油棕组织培养提供参考。The paper aims to study the influence of different plant hormones on the browning rate of explants during the tissue culture process of mature oil palm leaves. Taking the mature oil palm leaves as the explants,we treated them with different hormones as 2,4-D, NAA, IBA, IAA, KT, TDZ and 6-BA, to analyze the browning rate of oil palm leaves under induction. The results showed that:(1) the 6-BA and IBA had the highest browning rate for mature leaves than other kinds of plant hormones, and the browning rate was 66.98%and 65.35%, respectively;(2) the browning rate of mature leaves treated with KT was significantly lower than that treated with 6-BA, IBA and TDZ;(3) the browning rate of mature leaves treated with 2,4-D, NAA, IAA had no significant differences. It is suggested that KT should be given priority under the condition of similar callus induction rate.

关 键 词：油棕 植物激素 诱导愈伤 成熟叶片 褐化率 外植体 

分 类 号：S59[农业科学—作物学]