2种外源物质对大豆农艺性状及根际微生物群落的影响  

2 Exogenous Substances:Effects on Agronomic Traits and Rhizosphere Microflora of Soybean

作　　者：郝剑霞 王俊红 王梦亮[1] 刘雷 王星琳 Hao Jianxia;Wang Junhong;Wang Mengliang;Liu Lei;Wang Xinglin(Institute of Applied Chemistry,Shanxi University,Taiyuan 030006;Institute of Biotechnology,Shanxi University,Taiyuan 030006)

机构地区：[1]山西大学应用化学研究所,太原030006 [2]山西大学生物技术研究所,太原030006

出　　处：《中国农学通报》2019年第34期27-33,共7页Chinese Agricultural Science Bulletin

基　　金：国家重点研发计划子课题(2018YFD0200404-10);大田粮食作物生物配肥山西省高校协同创新中心(晋教科[2016]5号)

摘　　要：为了研究费氏中华根瘤菌(Sinorhizobium fredii)和二苯基脲磺酸钙(DSC)对大豆农艺性状和根际微生物群落的影响。采用盆栽方式,设置8个处理,通过对各组间大豆农艺性状的测定,选出具有代表性的组分进行群落结构多样性分析。结果表明,与CK相比,当DSC浓度为6 g/L时,全氮、全磷、豆荚数、豆粒数及株高显著提高,S.fredii+6 g/L DSC使根瘤鲜重、根瘤干重及根瘤数显著提高。因而选用CK、S.fredii(N1)、6 g/L DSC(N3)和S.fredii+6 g/L DSC(N6)的根际土壤进行高通量测序,结果显示:与CK相比,N1和N3群落多样性显著降低;N1益于Proteobacteria富集;N6益于Chloroflexi富集;N1、N3和N6益于Nitrospirae富集;N1、N3和N6较CK的Azoarcus、Azotobacter、Flavobacterium、Pseudomonas等功能菌属增加。综上,S.fredii和DSC处理有助于改善大豆各农艺性状指标,也影响着大豆的根际群落结构。To study the effects of Sinorhizobium fredii and calcium diphenylurea sulfonate(DSC) on agronomic traits and rhizosphere microflora of soybean, we set up 8 treatments by pot culture and selected representative components for the diversity analysis of community structure by measuring the agronomic traits of soybeans in each group. The results showed that: compared with CK, when DSC content was 6 g/L, the total nitrogen, total phosphorus, pod number, seed number and plant height were significantly increased;S.fredii + 6 g/L DSC significantly increased the fresh nodule weight, dry nodule weight and number of nodules, so we selected the rhizosphere soils of CK, S.fredii(N1), 6 g/L DSC(N3) and S.fredii + 6 g/L DSC(N6) for high-throughput sequencing, and the results showed that: the community diversity of N1 and N3 was significantly lower than CK;N1 was beneficial to the enrichment of Proteobacteria;N6 was beneficial to the enrichment of Chloroflexi;N1, N3 and N6 were beneficial to the enrichment of Nitrospirae;compared with CK, N1, N3 and N6 increased in Azoarcus, Azotobacter, Flavobacterium, Pseudomonas and other functional genus. In conclusion, S.fredii and DSC can improve the agronomic traits of soybean, and also affect the rhizosphere community structure of soybean.

关 键 词：大豆 根际微生物 高通量测序 费氏中华根瘤菌 二苯基脲磺酸钙 

分 类 号：S154.37[农业科学—土壤学]