利用SRAP引物组合构建甜菜品种的指纹图谱  

Establishing Sugarbeet DNA Fingerprint Based on SRAP Analysis

作　　者：闫彩燕 邹奕 马龙彪[1,2,3] 邳植 王茂芊[1,2,3] 兴旺 吴则东[1,2,3] Yan Caiyan;Zou Yi;Ma Longbiao;Pi Zhi;Wang Maoqian;Xing Wang;Wu Zedong(Key Laboratory of Sugar Beet Genetic Breeding/Heilongjiang University,Harbin 150080;Sugar Beet Research Institute of Chinese Academy of Agricultural Sciences/Crop Academy of Heilongjiang University,Harbin 150080;Sugarbeet Engineering Research Center of Heilongjiang Province,Harbin 150080)

机构地区：[1]黑龙江省普通高校甜菜遗传育种重点实验室/黑龙江大学,哈尔滨150080 [2]中国农业科学院甜菜研究所/黑龙江大学农作物研究院,哈尔滨150080 [3]黑龙江省甜菜工程技术研究中心,哈尔滨150080

出　　处：《中国农学通报》2019年第34期40-43,共4页Chinese Agricultural Science Bulletin

基　　金：国家糖料现代农业产业技术体系甜菜高品质品种改良岗位专项基金(CARS-170111);国家甜菜种质资源平台(NICGR2019-017);物种品种资源保护费(111821301354051001)

摘　　要：为构建甜菜品种的指纹图谱,对183对SRAP引物组合进行筛选,得到多态性好、条带清晰易于识别的SRAP引物组合2对(EM4-ME8和EM7-ME10)。利用筛选出的2对引物组合构建17份已经登记品种的指纹图谱,结果表明,引物组合EM7-ME10可以鉴别15个品种,扩增产生15种不同的带型;引物组合EM4-ME8可以鉴别14个品种,扩增产生14种不同的带型;这2对引物组合结合在一起可以鉴别全部的17个品种。利用SRAP引物组合构建甜菜品种指纹图谱的成功为快速鉴定甜菜品种的真实性提供了一种新的方法,也为保护农民和育种家的权益提供了思路。To construct the fingerprint of sugarbeet varieties, two pairs of SRAP primer combinations(EM4-ME8 and EM7-ME10) with good polymorphism and clear and easy identification were obtained by screening183 pairs of SRAP primer combinations. The fingerprints of 17 registered varieties were constructed by using the two pairs of primer combinations. The results showed that the primer combination EM7-ME10 could identify 15 varieties and amplify 15 different bands;the primer combination EM4-ME8 could identify 14 varieties and amplify 14 different bands;the two primer combinations combined could identify all 17 varieties.The success of using the SRAP primer combination to construct the fingerprint of sugarbeet varieties provides a new solution for the rapid identification of the authenticity of sugarbeet varieties, and provides a new way for protecting the rights of farmers and variety breeding professionals.

关 键 词：甜菜 指纹图谱 SRAP 分子标记技术 品种鉴定 

分 类 号：S566.3[农业科学—作物学]