抗逆转录病毒治疗对LAg-Avidity方法的影响  

The Effect of Antiretroviral Therapy on Limiting-antigen Avidity Enzyme Immunoassay

作　　者：杨洪[1] 李一平 苏玲[1] 陈晓茹 洪静 刘虎 梁姝[1] 肖琳[2] 杨文[1] YANG Hong;LI Yiping;SU Ling;CHEN Xiaoru;HONG Jing;LIU Hu;LIANG Shu;XIAO Lin;YANG Wen(Sichuan Center for Disease Control and Prevention,Chengdu 610041,Sichuan Province,China;Liangshan Prefecture Center for Disease Control and Prevention,Liangshan Prefecture 615000,Sichuan Province,China;Butuo County Center for Disease Control and Prevention,Liangshan Prefecture 615350,Sichuan Province,China)

机构地区：[1]四川省疾病预防控制中心,成都610041 [2]凉山州疾病预防控制中心,四川西昌615000 [3]布拖县疾病预防控制中心,四川布拖615350

出　　处：《预防医学情报杂志》2019年第12期1370-1373,共4页Journal of Preventive Medicine Information

基　　金：国家科技重大专项(项目编号：2018ZX10715-003-002)

摘　　要：目的研究抗逆转录病毒治疗对限制性抗原亲和力方法(Limiting-antigen avidity enzyme immunoassay,Lag-avidity EIA,简称LAg方法)的影响。方法 2017-10/2018-03收集凉山州布拖县木尔乡、九都乡和特木里镇434例接受抗逆转录病毒治疗病人血样,用LAg方法检测,分析CD4^+T淋巴细胞数、病毒载量数及抗逆转录病毒治疗时间对LAg方法误判的影响,率的比较采用卡方检验,以P<0.05为差异有统计学意义。结果 425例纳入分析的样本中,LAg方法误判为新近感染11例,误判比例3.59%(11/425),卡方分析发现随着CD4^+T淋巴细胞绝对计数值的增加,误判比例有所上升,上升趋势有统计学意义(χ^2=5.373,P=0.020);随着病毒载量值的增加,误判比例有所下降,下降趋势有统计学意义(χ^2=6.309,P=0.012);不同的治疗时间,误判比例有所不同,趋势变化无统计学意义(χ^2=0.162,P=0.688)。结论抗逆转录病毒治疗影响LAg-Avidity方法的误判比例,在横断面调查中应该把已开始抗病毒治疗的病人剔除;抗逆转录病毒治疗后,病毒载量值越低、CD4^+T淋巴细胞数越高的样本,越容易被LAg-Avidity方法误判为新近感染;LAg-Avidity方法对抗病毒治疗样本的误判比例不会随着治疗时间的延长而增加。Objective To study the effect of antiretroviral therapy on Limiting-antigen avidity enzyme immunoassay(LAg-avidity EIA). Methods LAg-avidity EIA was performed in 425 HIV-positiveserum samples from AIDS patients in Mu’er, Jiudu and Temuli Town, Butuo County, Liangshan Prefecture with their back information. CD4^+ lymphocyte count, viral load and the time of AntiRetroviral Therapy were analyzed to see the effect on LAg-avidity EIA. Chi-square test showed that there was significant difference when P(27) 0.05. Results There were 11 samples out of 425 samples misclassified as recently infected, and the rate of misclassification was 3.59%(11/425). Chi-square test showed that with the increase of CD4^+ lymphocyte count, the proportion of misclassification increased,and had the significant difference(χ^2=5.373,P=0.020);with the increase of viral load,the proportion of misclassification decreased, and had the significant difference( χ^2=6.309,P=0.012);with the time of Anti-Retroviral Therapy,the proportion of misclassification was different,and it had no significant difference(χ^2=0.162,P=0.688). Conclusion The Anti-Retroviral Therapy has an effect on the misclassification in Lag-avidity EIA,and HIV-positive people who have begun antiviral therapy should be excluded from cross-sectional surveys. After the Anti-Retroviral Therapy,samples with lower viral load and higher CD4^+ lymphocyte count were more likely to be misclassified as recently infected by LAg-avidity EIA. The misclassification proportion of LAg-avidity EIA will not increase with the time of Anti-Retroviral Therapy.

关 键 词：限制性抗原亲和力酶免法 HIV-1新发感染误判 抗逆转录病毒治疗 

分 类 号：R512.91[医药卫生—内科学]