益气化痰祛瘀方对慢性间歇缺氧模型大鼠肝脏及肠道微生物的影响  

Effect of Yiqi Huatan Quyu Recipe (益气化痰祛瘀方) on Liver and Intestinal Microorganism in Chronic Intermittent Hypoxia Model Rats

作　　者：伍娟 楼莹 陈沁 吴润华 王小婷 WU Juan;LOU Ying;CHEN Qin;WU Runhua;WANG Xiaoting(Clinical Skills Teaching Center of Fujian University of Traditional Chinese Medicine,Fuzhou,350122;College of Integrated Traditional Chinese and Western Medicine,Fujian University of Traditional Chinese Medicine)

机构地区：[1]福建中医药大学临床技能教学中心,福建省福州市350122 [2]福建中医药大学中西医结合学院

出　　处：《中医杂志》2019年第23期2050-2054,共5页Journal of Traditional Chinese Medicine

摘　　要：目的探讨益气化痰祛瘀方治疗阻塞性睡眠呼吸暂停低通气综合征肝损伤的可能作用机制。方法40只SD大鼠随机分为常氧对照组,模型组和益气化痰祛瘀低、高剂量组,每组10只。除常氧对照组外其余各组大鼠每天9:00-17:00置于低氧舱中共8周制备慢性间歇缺氧模型。造模第1天开始益气化痰祛瘀低、高剂量组分别于给予益气化痰祛瘀方19. 2、38. 4 ml/(kg·d)灌胃,常氧对照组和模型组给予2 ml生理盐水灌胃,共8周。HE染色观察大鼠肝组织病理变化,检测血清门冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)水平,16S多样性测序检测肠道微生物组成和相对丰度变化。结果模型组大鼠HE染色显示,肝细胞轻度水肿,肝组织炎症细胞浸润;血清AST和ALT较常氧对照组显著升高(P <0. 05)。益气化痰祛瘀高、低剂量组肝细胞呈条索状排列,炎症细胞减少,且益气化痰祛瘀高剂量组较低剂量组改善更明显,益气化痰祛瘀高剂量组血清AST和ALT亦较模型组降低(P <0. 05或P <0. 01)。16S多样性测序分析显示,常氧对照组特有微生物为310种,模型组112种,益气化痰祛瘀低剂量组126种,益气化痰祛瘀高剂量组138种。各组大鼠在门水平相对丰度前五的物种为:厚壁菌门、拟杆菌门、变形菌门、放线菌门、TM7菌门,各组大鼠组间各菌门组相对丰度差异无统计学意义(P> 0. 05)。与常氧对照组比较,模型组在属水平上乳酸杆菌、布劳特氏菌减少,颤螺菌属、别样棒菌属菌增多(P <0. 05);益气化痰祛瘀低、高剂量组乳杆菌属、布劳特氏菌属较模型组增多(P <0. 05)。结论益气化痰祛瘀方能影响肠道微生物种类、组成和丰度,调控短链脂肪酸的生成可能是其治疗阻塞性睡眠呼吸暂停低通气综合征肝损伤的机制之一。Objective To investigate the possible mechanism of Yiqi Huatan Quyu Recipe( 益气化痰祛瘀方) in treating liver injury of obstructive sleep apnea hypopnea syndrome( OSAHS). Methods Totally 40 SD rats were randomly divided into normoxic control group,model group,Yiqi Huatan Quyu Recipe low-dose group,and high-dose group,with 10 rats in each group. All groups but normoxic control group were placed in the hypoxic chamber for 8 weeks from 9: 00 to 17: 00 every day to prepare a chronic intermittent hypoxia model. On the first day of modeling,the Yiqi Huatan Quyu Recipe low-dose and high-dose groups were administered with Yiqi Huatan Quyu Recipe 19. 2,38. 4 ml/( kg·d) respectively,and the normoxic control group and the model group were given 2 ml normal saline by gavage for 8 weeks. HE staining was used to observe the pathological changes of rat liver tissue. Serum aspartate aminotransferase( AST) and alanine aminotransferase( ALT) levels were detected. 16 S diversity sequencing was used to detect intestinal microbial composition and relative abundance. Results In the model group,HE staining of the model group showed mild hepatic edema and inflammatory cell infiltration of liver tissue,serum AST and ALT were significantly increased compared with the normoxic control group( P < 0. 05). The hepatocytes in the Yiqi Huatan Quyu Recipe high-and low-dose groups were arranged in a strip-like shape,and the inflammatory cells were decreased. The improvement of the Yiqi Huatan Quyu Recipe high-dose group improved more obviously,and AST and ALT were also decreased compared with those of the model group( P < 0. 05 or P < 0. 01). 16 S diversity sequencing analysis showed that there were 310 endemic microorganisms in the normoxia control group,112 in the model group,126 in the Yiqi Huatan Quyu Recipe low-dose group,and 138 in the Yiqi Huatan Quyu Recipe high-dose group. The top 5 species in the relative abundance of the rats in each group were Firmicutes,Bacteroides,Proteobacteria,Actinobacteria,TM7,and there was no significan

关 键 词：益气化痰祛瘀方 阻塞性睡眠呼吸暂停低通气综合征 慢性间歇缺氧 肝损伤 肠道菌群 

分 类 号：R28[医药卫生—中药学]