Ubiquitin is double-phosphorylated by PINK1 for enhanced pH-sensitivity of conformational switch  

作　　者：Shang-XiangYe Zhou Gong Ju Yang Yu-Xin An Zhu Liu Qun Zhao Ewen Lescop Xu Dong Chun Tang 

机构地区：[1]Wuhan National Laboratory for Optoelectronics,Huazhong University of Science and Technology,Wuhan 430074,China [2]CAS Key Laboratory of Magnetic Resonance in Biological Systems,State Key Laboratory of Magnetic Resonance and Atomic Molecular Physics,National Center for Magnetic Resonance at Wuhan,Wuhan Institute of Physics and Mathematics of the Chinese Academy of Sciences,Wuhan 430071,China [3]Institut de Chimie des Substances Naturelles,CNRS UPR 2301,Univ Paris-Sud,Universite Paris-Saclay,91198 Gif-sur-Yvette cedex,France [4]CAS Key Laboratory of Separation Sciences for Analytical Chemistry,Dalian Institute of Chemical Physics of the Chinese Academy of Sciences,Dalian 116023,China

出　　处：《Protein & Cell》2019年第12期908-913,共6页蛋白质与细胞（英文版）

基　　金：The work has been supported by the National Key R&D Program of China(2018YFA0507700 to C.Tang,Z.Liu,Z.Gong and Q.Zhao;2017YFA0505400 to X.Dong;2016YFA0501200 to C.Tang,Z.Gong and X.Dong);by the National Natural Science Foundation of China(91753132 and 31770799 to C.Tang).

摘　　要：Dear Editor,Multisite phosphorylation is observed in many signaling proteins,which confers the proteins new functions.A multi­site phosphorylated protein can exhibit switch-like response to cellular stimuli,with the often-clustered phosphorylation sites either unphosphorylated or fully phosphorylated by the same kinase(Kapuy et al..2009).Multisite phosphorylation has also been reported to promote the folding of an intrin­sically disordered protein,and consequently modulate the binding affinity to other proteins(Bah et al.,2015).Here we show that multisite phosphorylation makes a protein a better pH sensor,promptly switching between alternative confor­mational states in response to pH change under physiolog­ical conditions.

关 键 词：PROMPT FOLDING 

分 类 号：R38[医药卫生—医学寄生虫学]