小粒野生稻OmSKP1基因的克隆与功能初探  

Cloning and Functional Analysis of OmSKP1 Gene from Oryza minuta

作　　者：吴渊源 韦鹏飞 郭崇炎 范锡麟 熊佳旺 王志龙 陈秋红 Wu􀀁Yuanyuan;Wei􀀁Pengfei;Guo􀀁Chongyan;Fan􀀁Xilin;Xiong􀀁Jiawang;Wang􀀁Zhilong;Chen􀀁Qiuhong(Crop Gene Engineering Key Lab of Hunan Province,Hu'nan Provincial Key Laboratory of Rice and Rapseed Breeding for Disease Resistance,College of Agronomy,Hu'nan Agricultural University,Changsha,410128)

机构地区：[1]湖南农业大学农学院水稻油菜抗病育种湖南省重点实验室作物基因工程湖南省重点实验室

出　　处：《分子植物育种》2019年第23期7641-7648,共8页Molecular Plant Breeding

基　　金：国家自然科学基金项目(31401699; 3157995);湖南省自然科学基金项目(2017JJ3112)共同资助

摘　　要：S期激酶相关蛋白1(SKPl)是E3泛素蛋白连接酶SCF复合物的核心组成元件。为探索水稻中SKP1蛋白的功能,本研究从小粒野生稻(Oryza minuta)中克隆了OmSKP1基因,获得了该基因的全长CDS。生物信息学分析结果表明,OmSKP1具有典型的SKP1蛋白结构域,是一个亲水性蛋白。农艺性状分析结果表明,OmSKP1过表达转基因水稻植株的株高、穗长、每穗实粒数、千粒重均低于背景材料日本晴(NPB),但其有效穗数高于NPB。稻瘟菌接种试验表明,OmSKP1过表达转基因水稻植株相对于对照日本晴表现出更感病的表型。本研究为野生稻OmSKP1基因功能的深入解析提供科学依据。S phase kinase-associated protein 1(SKPl)is a core component of SCF complex.In order to explore the function of SKP1 protein in rice,OmSKP1 gene with full-length CDS from wild rice(Oryza minuta)was cloned.Bioinformatic analysis results showed that OmSKP1 protein contains a typical SKP1 protein domain,and is hydrophilic.Agronomic traits analysis results showed that the plant height,panicle length,number of grains per panicle and1000-grain weight of the transgenic plants over-expressing OmSKP1 were lower than that of the wild type(Nipponbare NPB)plants,but the number of productive panicles of the transgenic plants was more than that of NPB plants.Rice blast inoculation test showed that OmSKP1 over-expressed plants displayed a more susceptible phenotype compared to wild type plants.This study provides a basis for the further functional exploration of the OmSKP1 gene.

关 键 词：野生稻 OmSKP1 生物信息学 稻瘟病 

分 类 号：S51[农业科学—作物学]