食管鳞状细胞癌血液中循环肿瘤DNA(ctDNA)含量的检测及其临床学意义  

Detection of circulating tumor DNA(ctDNA) in the blood of esophageal squamous cell carcinoma and its clinical significance

作　　者：殷文明[1] 茅爱武[2] 姚辉 徐国平[1] 顾文栋[1] YIN Wen-ming;MAO Ai-wu;YAO Hui;XU Guo-ping;GU Wen-dong(Changzhou First People's Hospital,Jiangsu,213003,China)

机构地区：[1]苏州大学附属第三医院,江苏213003 [2]上海交通大学医学院附属同仁医院

出　　处：《中国地方病防治》2019年第5期492-493,共2页Chinese Journal of Control of Endemic Diseases

基　　金：国家自然科学基金面上项目(81571775);希思科临床肿瘤学研究基金会资助项目(Y-HR2018-063)

摘　　要：目的本文主要探讨血液中ctDNA含量(小DNA,完整DNA和拷贝数)与肿瘤预后(复发和死亡)的相关性。方法本研究采集常州第一人民医院2017年9月到2018年4月的100例经病理学检查证实为食管鳞状细胞癌患者的静脉血,荧光定量PCR检测血浆中ctDNA的含量,数字PCR(dPCR)检测基因拷贝数变异,并随访跟踪患者预后,复发及死亡情况。结果在ctDNA的三种分析指标中,只有完整DNA与患者的预后及死亡具有相关性(P<0.05)。结论 ctDNA中完整DNA的含量高低对食管癌患者的预后及存活具有临床指导意义。Objective This article mainly discusses ctDNA content in the blood(small DNA,complete DNA and DNA copy number) and the correlation between ctDNA and tumor prognosis(recurrence and death).Methods A total of 100 cases of patients with esophageal squamous cell carcinoma who were confirmed as esophageal squamous cell carcinoma by pathological examination were collected from September 2017 to April 2018 in the Changzhou First People’s Hospital.The content of ctDNA in plasma was detected by fluorescence quantitative PCR,and gene copy number was detected by digital PCR.Results Complete DNA content was associated with prognosis and death of patients(P<0.05).Conclusion The content of complete DNA in blood has clinical significance for the prognosis and survival of patients with esophageal cancer.

关 键 词：食管鱗状细胞癌 循环肿瘤DNA 复发及生存 

分 类 号：R446[医药卫生—诊断学]