基于抑菌效应成分指纹谱的黄芩质量评价研究  

Establishment of an antibacterially effective components fingerprint for quality evaluation of Scutellariae Radix(Huangqin)

作　　者：任永申[1] 邓鑫 雷蕾 梁帅 郑尧 艾娇 李燕[1] 廖矛川[1] REN Yong-shen;DENG Xin;LEI Lei;LIANG Shuai;ZHENG Yao;AI Jiao;LI Yan;LIAO Mao-chuan(School of Pharmaceutical Science,South-central University For Nationalities,Wuhan 430070,China)

机构地区：[1]中南民族大学药学院

出　　处：《药学学报》2019年第12期2155-2161,共7页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金资助项目(81202935,81773893);国家“重大新药创制”科技重大专项资助项目(2017ZX09301060-001);国家重点研发计划“中医药现代化研究”课题资助(2017YFC1701004)

摘　　要：化学指纹图谱是中药质控的重要手段,但常规指纹图谱存在冗余信息,影响了其准确性和可用性,需要进行改进和提升。本研究采用量反应平行线法原理检测黄芩抑菌活性生物效价;采用HPLC法检测不同黄芩样品的化学指纹图谱;采用灰色关联法(GRA)辨识主要抑菌效应成分。结果表明,15批黄芩样品抑菌效价在0~1000 U·g^-1,平均抑菌效价为556.29±258.57 U·g^-1(1 U约相当于2.25μg庆大霉素的抑菌活性);15批黄芩样品的HPLC指纹图谱中共标定34个特征峰,样品间相似度为0.255~0.991;取灰色关联序前10位成分且与抑菌效价成正相关的P33、P30(黄芩素)、P19(黄芩苷)、P15、P29、P34、P31(汉黄芩素)、P28的8个成分并赋予权重,建立黄芩效应成分指纹谱,能够明确区分黄芩各规格等级样品,并能综合表征黄芩性状、成分、抑菌效价差异。综之,本研究构建了直接关切黄芩抑菌活性且信息简化的抑菌效应成分指纹谱,可综合评价黄芩质量,提高了中药指纹图谱的功效专属性和质量辨识能力,并可为其他中药质量综合评价提供有益参考。A chemical fingerprint is an important mean for quality control of traditional Chinese medicine(TCM);however,there is much redundant information in a conventional fingerprint that can affect its availability and accuracy.In this work,the antibacterial biopotency of Scutellariae Radix(Huangqin,HQ)was determined according to the parallel line method of quantitative response.HPLC was adopted to detect the chemical fingerprint of HQ;Grey relational analysis(GRA)was used to identify the primary effective components.The results showed that the antibacterial biopotency of 15 batches of HQ ranged from 0 to 1000 U·g^-1 and the average potency was 556.29±258.57 U·g^-1(1 U is equivalent to the bacteriostatic activity of 2.25μg gentamicin).There were 34 characteristic peaks in the fingerprints of the samples and their similarities were 0.255-0.991.Eight components(P33,P30/baicalein,P19/baicalin,P15,P29,P34,P31/wogonin and P28)are positively related to antibacterial biopotency and selected from the top ten components of the grey correlation sequence to define the antibacterially effective components fingerprint of HQ.This fingerprint can clearly distinguish the commodity specification and grade,and can also characterize the morphology,components and the bacteriostatic potency differences of HQ.In summary,we established an antibacterially effective components fingerprint which provides simplified information on the antibacterial activity of Scutellariae Radix and could significantly improve the efficacy,specificity,and discriminative ability of the fingerprint for HQ,and could be a useful reference for the comprehensive quality evaluation of other TCM.

关 键 词：黄芩 生物效价检测 灰色关联分析 抑菌效应成分指纹谱 

分 类 号：R917[医药卫生—药物分析学]